Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 73022..73569 Replicon plasmid pRHB32-C22_2
Accession NZ_CP057221
Organism Escherichia fergusonii strain RHB32-C22

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag HVZ07_RS22395 Protein ID WP_000969996.1
Coordinates 73022..73303 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag HVZ07_RS22400 Protein ID WP_000079941.1
Coordinates 73300..73569 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ07_RS22380 69315..69680 + 366 WP_097418983.1 transposase -
HVZ07_RS22385 69680..70863 + 1184 Protein_65 IS91 family transposase -
HVZ07_RS22390 71046..72542 - 1497 Protein_66 hypothetical protein -
HVZ07_RS22395 73022..73303 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HVZ07_RS22400 73300..73569 - 270 WP_000079941.1 hypothetical protein Antitoxin
HVZ07_RS22405 74483..75340 - 858 WP_097418981.1 incFII family plasmid replication initiator RepA -
HVZ07_RS22410 75333..75815 - 483 WP_001523473.1 hypothetical protein -
HVZ07_RS22415 75808..75882 - 75 WP_001442103.1 RepA leader peptide Tap -
HVZ07_RS22420 76114..76371 - 258 WP_000083845.1 replication regulatory protein RepA -
HVZ07_RS22425 76655..76861 - 207 WP_001351576.1 type I toxin-antitoxin system Hok family toxin -
- 76848..76909 + 62 NuclAT_1 - -
- 76848..76909 + 62 NuclAT_1 - -
- 76848..76909 + 62 NuclAT_1 - -
- 76848..76909 + 62 NuclAT_1 - -
HVZ07_RS22430 77492..77704 - 213 WP_097418979.1 hypothetical protein -
HVZ07_RS22435 77839..78399 - 561 WP_097418978.1 fertility inhibition protein FinO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyC 1..147240 147240


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T164993 WP_000969996.1 NZ_CP057221:c73303-73022 [Escherichia fergusonii]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T164993 NZ_CP073627:323452-323820 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT164993 WP_000079941.1 NZ_CP057221:c73569-73300 [Escherichia fergusonii]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT164993 NZ_CP073627:323234-323455 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References