Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 725457..726007 Replicon chromosome
Accession NZ_CP057220
Organism Escherichia fergusonii strain RHB32-C22

Toxin (Protein)


Gene name ccdB Uniprot ID F0JPU1
Locus tag HVZ07_RS03550 Protein ID WP_001160963.1
Coordinates 725693..726007 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HVZ07_RS03545 Protein ID WP_000125566.1
Coordinates 725457..725690 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ07_RS03520 720530..720817 + 288 WP_000203741.1 ferredoxin-like protein FixX -
HVZ07_RS03525 720876..722207 + 1332 WP_074500872.1 MFS transporter -
HVZ07_RS03530 722315..722845 + 531 WP_000600701.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HVZ07_RS03535 722838..724700 + 1863 WP_181668156.1 glutathione-regulated potassium-efflux system protein KefC -
HVZ07_RS03540 724892..725371 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HVZ07_RS03545 725457..725690 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HVZ07_RS03550 725693..726007 + 315 WP_001160963.1 CcdB family protein Toxin
HVZ07_RS03555 726004..726852 - 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HVZ07_RS03560 726859..727236 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HVZ07_RS03565 727239..728060 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HVZ07_RS03570 728057..729046 - 990 WP_181668157.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HVZ07_RS03575 729046..730332 - 1287 WP_181668158.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11722.70 Da        Isoelectric Point: 8.0666

>T164976 WP_001160963.1 NZ_CP057220:725693-726007 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T164976 NZ_CP073624:1908164-1908415 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT164976 WP_000125566.1 NZ_CP057220:725457-725690 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT164976 NZ_CP073624:1908412-1908666 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F0JPU1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References