Detailed information of TA system TA164955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4013850..4014426
• Replicon: chromosome
• Accession: NZ_CP057215
• Organism: Escherichia fergusonii strain RHB33-C04

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HVZ08_RS19480
• Protein ID: WP_181666838.1
• Coordinates: 4014139..4014426 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A7U9DAW4
• Locus tag: HVZ08_RS19475
• Protein ID: WP_000063150.1
• Coordinates: 4013850..4014152 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ08_RS19455	4009664..4010176	+	513	WP_001175455.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-
HVZ08_RS19460	4010446..4012596	+	2151	WP_001314407.1	pyruvate/proton symporter BtsT	-
HVZ08_RS19465	4012646..4012849	+	204	WP_000467859.1	YbdD/YjiX family protein	-
HVZ08_RS19470	4012860..4013816	+	957	WP_001295525.1	GTPase	-
HVZ08_RS19475	4013850..4014152	-	303	WP_000063150.1	BrnA antitoxin family protein	Antitoxin
HVZ08_RS19480	4014139..4014426	-	288	WP_181666838.1	BrnT family toxin	Toxin
HVZ08_RS19485	4014623..4015789	+	1167	WP_000800829.1	restriction endonuclease	-
HVZ08_RS19490	4015853..4017472	+	1620	WP_181666839.1	type I restriction-modification system subunit M	-
HVZ08_RS19495	4017462..4018199	+	738	Protein_3801	restriction endonuclease subunit S	-
HVZ08_RS19500	4018426..4019123	-	698	Protein_3802	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4018848..4019123	275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11188.68 Da
• Isoelectric Point: 7.4697

>T164955 WP_181666838.1 NZ_CP057215:c4014426-4014139 [Escherichia fergusonii]
MPMEFEWDANKAKSNRMKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T164955 NZ_CP073621:c2949263-2948799 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11532.03 Da
• Isoelectric Point: 9.4646

>AT164955 WP_000063150.1 NZ_CP057215:c4014152-4013850 [Escherichia fergusonii]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT164955 NZ_CP073621:c2949598-2949263 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9DAW4