Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 993779..994362 Replicon chromosome
Accession NZ_CP057215
Organism Escherichia fergusonii strain RHB33-C04

Toxin (Protein)


Gene name mazF Uniprot ID B7LWP2
Locus tag HVZ08_RS04805 Protein ID WP_000254732.1
Coordinates 994027..994362 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HVZ08_RS04800 Protein ID WP_000581937.1
Coordinates 993779..994027 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ08_RS04790 990118..991416 + 1299 WP_000046872.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
HVZ08_RS04795 991467..993701 + 2235 WP_181667157.1 GTP diphosphokinase -
HVZ08_RS04800 993779..994027 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HVZ08_RS04805 994027..994362 + 336 WP_000254732.1 endoribonuclease MazF Toxin
HVZ08_RS04810 994433..995224 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HVZ08_RS04815 995452..997089 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HVZ08_RS04820 997177..998475 + 1299 WP_181667158.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12156.08 Da        Isoelectric Point: 7.7128

>T164938 WP_000254732.1 NZ_CP057215:994027-994362 [Escherichia fergusonii]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSDQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T164938 NZ_CP073590:30432-30737 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCTGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT164938 WP_000581937.1 NZ_CP057215:993779-994027 [Escherichia fergusonii]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT164938 NZ_CP073590:30212-30430 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B7LWP2

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References