Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 753944..754494 Replicon chromosome
Accession NZ_CP057215
Organism Escherichia fergusonii strain RHB33-C04

Toxin (Protein)


Gene name ccdB Uniprot ID F0JPU1
Locus tag HVZ08_RS03720 Protein ID WP_001160963.1
Coordinates 754180..754494 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HVZ08_RS03715 Protein ID WP_000125566.1
Coordinates 753944..754177 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ08_RS03690 749016..749303 + 288 WP_000203741.1 ferredoxin-like protein FixX -
HVZ08_RS03695 749362..750693 + 1332 WP_181667119.1 MFS transporter -
HVZ08_RS03700 750801..751331 + 531 WP_181667120.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HVZ08_RS03705 751324..753186 + 1863 WP_181667121.1 glutathione-regulated potassium-efflux system protein KefC -
HVZ08_RS03710 753379..753858 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HVZ08_RS03715 753944..754177 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HVZ08_RS03720 754180..754494 + 315 WP_001160963.1 CcdB family protein Toxin
HVZ08_RS03725 754491..755339 - 849 WP_000257179.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HVZ08_RS03730 755346..755723 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HVZ08_RS03735 755726..756547 - 822 WP_001065390.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HVZ08_RS03740 756544..757533 - 990 WP_046076786.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HVZ08_RS03745 757533..758819 - 1287 WP_181667122.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11722.70 Da        Isoelectric Point: 8.0666

>T164937 WP_001160963.1 NZ_CP057215:754180-754494 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T164937 NZ_CP073589:c4537552-4537334 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT164937 WP_000125566.1 NZ_CP057215:753944-754177 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT164937 NZ_CP073589:c4537952-4537578 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F0JPU1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References