Detailed information of TA system TA164914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4013805..4014381
• Replicon: chromosome
• Accession: NZ_CP057210
• Organism: Escherichia fergusonii strain RHB33-C07

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HVZ09_RS19515
• Protein ID: WP_181666838.1
• Coordinates: 4014094..4014381 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A7U9DAW4
• Locus tag: HVZ09_RS19510
• Protein ID: WP_000063150.1
• Coordinates: 4013805..4014107 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ09_RS19490	4009619..4010131	+	513	WP_001175455.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-
HVZ09_RS19495	4010401..4012551	+	2151	WP_001314407.1	pyruvate/proton symporter BtsT	-
HVZ09_RS19500	4012601..4012804	+	204	WP_000467859.1	YbdD/YjiX family protein	-
HVZ09_RS19505	4012815..4013771	+	957	WP_001295525.1	GTPase	-
HVZ09_RS19510	4013805..4014107	-	303	WP_000063150.1	BrnA antitoxin family protein	Antitoxin
HVZ09_RS19515	4014094..4014381	-	288	WP_181666838.1	BrnT family toxin	Toxin
HVZ09_RS19520	4014578..4015744	+	1167	WP_000800829.1	restriction endonuclease	-
HVZ09_RS19525	4015808..4017427	+	1620	WP_181666839.1	type I restriction-modification system subunit M	-
HVZ09_RS19530	4017417..4018154	+	738	Protein_3808	restriction endonuclease subunit S	-
HVZ09_RS19535	4018381..4019078	-	698	Protein_3809	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4018803..4019078	275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11188.68 Da
• Isoelectric Point: 7.4697

>T164914 WP_181666838.1 NZ_CP057210:c4014381-4014094 [Escherichia fergusonii]
MPMEFEWDANKAKSNRMKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T164914 NZ_CP073587:1200070-1200339 [Shewanella yunxiaonensis]
ATGAGTAGTCGTCAACATATACTGTCCTGGACTGATGACGCTTGGGAGGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAATAAACTTATTAATGATGTTAAGCGCTCTCCGTTTGAGGGGATTGGTAAACCTGAACCTTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTATTGATGACACCAATAGGCTTGTTTACGTAGTCGATGATCAAGCTATA
ACGATAATTTCTTGTCGTTATCACTACTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11532.03 Da
• Isoelectric Point: 9.4646

>AT164914 WP_000063150.1 NZ_CP057210:c4014107-4013805 [Escherichia fergusonii]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT164914 NZ_CP073587:1199823-1200077 [Shewanella yunxiaonensis]
GTGAAAATCGTATCTTTTACTGAAGCTAGAAATGGTCTCAAAGCTGTTTTAGACGGTGTAGTTAATGATGCTGATACTAC
AGTTATTACGCGCCGCGATTCTGAAGATGCAGTGGTTATGTCTTTAGATTATTACAATAGCCTTATGGAGACAGTTCACT
TACTACGTTCTCCTCAAAATGCTGAACACCTAAACCGTTCGATAGCACAGTACCGAGCTGGTAAAACAACAGCACGTGAG
TTAATTGATGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9DAW4