Detailed information of TA system TA164876

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 91284..91963
• Replicon: chromosome
• Accession: NZ_CP057150
• Organism: Citrobacter sp. RHB35-C17

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A7L6UCB7
• Locus tag: HVZ37_RS00425
• Protein ID: WP_042998666.1
• Coordinates: 91284..91625 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A7L6UB12
• Locus tag: HVZ37_RS00430
• Protein ID: WP_042998665.1
• Coordinates: 91646..91963 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ37_RS00400	86633..87349	+	717	WP_042998668.1	GntR family transcriptional regulator	-
HVZ37_RS00405	87545..88522	+	978	WP_084196634.1	SIS domain-containing protein	-
HVZ37_RS00410	88586..89431	+	846	WP_042323385.1	fructoselysine 6-kinase	-
HVZ37_RS00415	89493..90812	+	1320	WP_042323383.1	anaerobic C4-dicarboxylate transporter	-
HVZ37_RS00420	91023..91181	-	159	WP_155985518.1	hypothetical protein	-
HVZ37_RS00425	91284..91625	-	342	WP_042998666.1	TA system toxin CbtA family protein	Toxin
HVZ37_RS00430	91646..91963	-	318	WP_042998665.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HVZ37_RS00435	91981..92202	-	222	WP_072031697.1	DUF987 domain-containing protein	-
HVZ37_RS00440	92211..92693	-	483	WP_032250314.1	DNA repair protein RadC	-
HVZ37_RS00445	92702..93163	-	462	WP_016239686.1	antirestriction protein	-
HVZ37_RS00450	93177..93368	-	192	Protein_89	DUF905 family protein	-
HVZ37_RS00455	93666..93800	-	135	Protein_90	arsenical resistance protein ArsH	-
HVZ37_RS00460	93880..94284	-	405	WP_024195748.1	H-NS histone family protein	-
HVZ37_RS00465	94810..95037	-	228	WP_016239708.1	hypothetical protein	-
HVZ37_RS00470	95338..95556	-	219	WP_016239707.1	cold shock domain-containing protein	-
HVZ37_RS00475	95823..96035	+	213	WP_001446195.1	cold shock-like protein CspF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12790.75 Da
• Isoelectric Point: 8.9789

>T164876 WP_042998666.1 NZ_CP057150:c91625-91284 [Citrobacter sp. RHB35-C17]
MKPLPATTSRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFSDETVIQENIDAGITLADAVNFLVEKYELVRIDRNGF
SWQEQSPYLRAVDILRARQATGLLQRNRKHAAL

Nucleotide

• Length: 342 bp

>T164876 NZ_CP073365:1324368-1324718 [Paenibacillus tritici]
TTGATTGTTAAACGCGGCGACGTTTTTTTTGCCGACCTTTCACCGGTTGTAGGTTCTGAGCAAGGTGGAGTAAGGCCGGT
ACTGGTCATTCAGAACGATATTGGCAATCGCTTCAGCCCGACTGTGATTGTGGCAGCTATCACTGCCCAGATCCAGAAGG
CCAAACTGCCGACGCATGTAGAGATTGATGCGGCTGCACATGGCTTTGACCGGGATTCCGTGATCTTGCTGGAGCAGGTT
CGGACAATTGACAAACAACGCTTGACCGATAAGATCACCCACCTGGACGATGAGACCATGAAGCTGGTGGATGATTCGCT
GCAAATCAGCCTGGGGCTGATTGATTTCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11925.51 Da
• Isoelectric Point: 7.7715

>AT164876 WP_042998665.1 NZ_CP057150:c91963-91646 [Citrobacter sp. RHB35-C17]
MNNTTWGLQRHISPRLGARLVQEGNRLHYLADRASITGRFSDAECRKLDETFPHFISQMESILTTGELNPRHAHCVTLYH
NGFTCKADTLGNCGYVYIAIYPTQL

Nucleotide

• Length: 318 bp

>AT164876 NZ_CP073365:1324083-1324364 [Paenibacillus tritici]
GTGGCCAATTTGCAGAACACCAAAAGAATCATGATCAGCTTGCCCGATCATCTCTTGCAGGAAGTAGATGGGATCGTTCA
ATTGGAGAACTCTAACCGTAGTGAATTGATCAGGCAGGCCATGAAGCTGTATCTGAGCGACCGGAGGAAACGTACCATCC
GTGAGTCCATGCAGCGTGGCTATATGGAAATGGCTAAGATTAATTTGACCATGGCATGCGAGGCCTTTCTCGCGGAGGAA
GATGCAGACAGTACTCTTGGCCGCTTAGTAAGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A7L6UCB7

Antitoxin

• AlphaFold DB: A0A7L6UB12