Detailed information of TA system TA164875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 214354..215255
• Replicon: plasmid pRHB35-C21_2
• Accession: NZ_CP057148
• Organism: Citrobacter sp. RHB35-C21

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A837LJP8
• Locus tag: HVZ39_RS26170
• Protein ID: WP_007372300.1
• Coordinates: 214354..214812 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HVZ39_RS26175
• Protein ID: WP_008786588.1
• Coordinates: 214809..215255 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ39_RS26125	210080..210322	+	243	WP_007372291.1	hypothetical protein	-
HVZ39_RS26130	210333..210731	+	399	WP_007372292.1	hypothetical protein	-
HVZ39_RS26135	210815..211141	+	327	WP_007372293.1	hypothetical protein	-
HVZ39_RS26140	211504..211842	-	339	WP_007372294.1	hypothetical protein	-
HVZ39_RS26145	212080..212523	+	444	WP_007372295.1	hypothetical protein	-
HVZ39_RS26150	212642..212983	+	342	WP_007372296.1	hypothetical protein	-
HVZ39_RS26155	213142..213696	+	555	WP_007372297.1	hypothetical protein	-
HVZ39_RS26160	213746..214012	+	267	WP_007372298.1	hypothetical protein	-
HVZ39_RS26165	214080..214310	-	231	WP_007372299.1	hypothetical protein	-
HVZ39_RS26170	214354..214812	-	459	WP_007372300.1	RES family NAD+ phosphorylase	Toxin
HVZ39_RS26175	214809..215255	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
HVZ39_RS26180	215404..215721	+	318	WP_007372302.1	hypothetical protein	-
HVZ39_RS26185	215763..216605	+	843	WP_007372303.1	hypothetical protein	-
HVZ39_RS26190	216577..216855	+	279	WP_007372304.1	hypothetical protein	-
HVZ39_RS26195	216915..217358	+	444	WP_007372305.1	hypothetical protein	-
HVZ39_RS26200	217645..217809	+	165	WP_007372306.1	hypothetical protein	-
HVZ39_RS26205	218377..219219	+	843	Protein_265	HNH endonuclease	-
HVZ39_RS26210	219641..219892	+	252	WP_024196080.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..252642	252642

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16975.40 Da
• Isoelectric Point: 4.5730

>T164875 WP_007372300.1 NZ_CP057148:c214812-214354 [Citrobacter sp. RHB35-C21]
VILYRLTKTRYLMTAWSGLGAKEAGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T164875 NZ_CP073362:c106916-106518 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT164875 WP_008786588.1 NZ_CP057148:c215255-214809 [Citrobacter sp. RHB35-C21]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT164875 NZ_CP073362:c107143-106916 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A837LJP8