Detailed information of TA system TA164874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 207055..207605
• Replicon: plasmid pRHB35-C21_2
• Accession: NZ_CP057148
• Organism: Citrobacter sp. RHB35-C21

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: HVZ39_RS26100
• Protein ID: WP_007372286.1
• Coordinates: 207297..207605 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: HVZ39_RS26095
• Protein ID: WP_007372285.1
• Coordinates: 207055..207294 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ39_RS26050	202062..202457	+	396	WP_024196081.1	hypothetical protein	-
HVZ39_RS26055	202639..202830	+	192	WP_016241560.1	hypothetical protein	-
HVZ39_RS26060	202961..203656	+	696	WP_007372278.1	hypothetical protein	-
HVZ39_RS26065	203679..203876	+	198	WP_032155220.1	Lar family restriction alleviation protein	-
HVZ39_RS26070	203929..204387	+	459	WP_008786599.1	hypothetical protein	-
HVZ39_RS26075	204535..204675	+	141	WP_007372281.1	hypothetical protein	-
HVZ39_RS26080	204691..205323	+	633	WP_007372282.1	hypothetical protein	-
HVZ39_RS26085	205784..206455	+	672	WP_007372283.1	hypothetical protein	-
HVZ39_RS26090	206452..206916	+	465	WP_008786596.1	hypothetical protein	-
HVZ39_RS26095	207055..207294	+	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HVZ39_RS26100	207297..207605	+	309	WP_007372286.1	CcdB family protein	Toxin
HVZ39_RS26105	207626..207784	+	159	WP_016241558.1	hypothetical protein	-
HVZ39_RS26110	207939..208493	+	555	WP_007372288.1	hypothetical protein	-
HVZ39_RS26115	208563..209180	+	618	WP_007372289.1	hypothetical protein	-
HVZ39_RS26120	209263..209733	+	471	WP_007372290.1	hypothetical protein	-
HVZ39_RS26125	210080..210322	+	243	WP_007372291.1	hypothetical protein	-
HVZ39_RS26130	210333..210731	+	399	WP_007372292.1	hypothetical protein	-
HVZ39_RS26135	210815..211141	+	327	WP_007372293.1	hypothetical protein	-
HVZ39_RS26140	211504..211842	-	339	WP_007372294.1	hypothetical protein	-
HVZ39_RS26145	212080..212523	+	444	WP_007372295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..252642	252642

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T164874 WP_007372286.1 NZ_CP057148:207297-207605 [Citrobacter sp. RHB35-C21]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T164874 NZ_CP073362:c64505-64356 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT164874 WP_007372285.1 NZ_CP057148:207055-207294 [Citrobacter sp. RHB35-C21]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT164874 NZ_CP073362:64549-64610 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3