Detailed information of TA system TA164872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4976731..4977347
• Replicon: chromosome
• Accession: NZ_CP057147
• Organism: Citrobacter sp. RHB35-C21

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HVZ39_RS24040
• Protein ID: WP_016155182.1
• Coordinates: 4976731..4977105 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: R8WIS4
• Locus tag: HVZ39_RS24045
• Protein ID: WP_016155181.1
• Coordinates: 4977105..4977347 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ39_RS24025	4974234..4975136	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
HVZ39_RS24030	4975133..4975768	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HVZ39_RS24035	4975765..4976694	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HVZ39_RS24040	4976731..4977105	-	375	WP_016155182.1	type II toxin-antitoxin system VapC family toxin	Toxin
HVZ39_RS24045	4977105..4977347	-	243	WP_016155181.1	ribbon-helix-helix domain-containing protein	Antitoxin
HVZ39_RS24050	4977553..4978482	+	930	WP_181633048.1	alpha/beta hydrolase	-
HVZ39_RS24055	4978567..4978878	+	312	WP_019077936.1	type II toxin-antitoxin system HigB family toxin	-
HVZ39_RS24060	4978875..4979327	+	453	WP_181633049.1	helix-turn-helix domain-containing protein	-
HVZ39_RS24065	4979345..4980286	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HVZ39_RS24070	4980331..4980768	-	438	WP_181633050.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HVZ39_RS24075	4980765..4981637	-	873	WP_019077938.1	virulence factor BrkB family protein	-
HVZ39_RS24080	4981631..4982230	-	600	WP_016155174.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13678.84 Da
• Isoelectric Point: 8.5373

>T164872 WP_016155182.1 NZ_CP057147:c4977105-4976731 [Citrobacter sp. RHB35-C21]
MVTGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQESRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T164872 NZ_CP073360:c4672456-4672145 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.72 Da
• Isoelectric Point: 4.3331

>AT164872 WP_016155181.1 NZ_CP057147:c4977347-4977105 [Citrobacter sp. RHB35-C21]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Nucleotide

• Length: 243 bp

>AT164872 NZ_CP073360:c4672144-4671854 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: R8WIS4