Detailed information of TA system TA164871

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 4678241..4679034
• Replicon: chromosome
• Accession: NZ_CP057147
• Organism: Citrobacter sp. RHB35-C21

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: HVZ39_RS22715
• Protein ID: WP_000854726.1
• Coordinates: 4678657..4679034 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: HVZ39_RS22710
• Protein ID: WP_001548930.1
• Coordinates: 4678241..4678567 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ39_RS22665	4673679..4674500	+	822	WP_042111319.1	DUF945 domain-containing protein	-
HVZ39_RS22670	4674717..4675418	+	702	WP_042111317.1	WYL domain-containing protein	-
HVZ39_RS22675	4675459..4675695	+	237	WP_001144031.1	hypothetical protein	-
HVZ39_RS22680	4675695..4676138	+	444	WP_000649865.1	hypothetical protein	-
HVZ39_RS22685	4676161..4676628	+	468	WP_001385283.1	hypothetical protein	-
HVZ39_RS22690	4676705..4676944	+	240	WP_000194654.1	DUF905 domain-containing protein	-
HVZ39_RS22695	4677042..4677500	+	459	WP_000211838.1	antirestriction protein	-
HVZ39_RS22700	4677516..4677992	+	477	WP_000811693.1	RadC family protein	-
HVZ39_RS22705	4678001..4678222	+	222	WP_000691994.1	DUF987 domain-containing protein	-
HVZ39_RS22710	4678241..4678567	+	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
HVZ39_RS22715	4678657..4679034	+	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
HVZ39_RS22720	4679031..4679519	+	489	WP_001547769.1	hypothetical protein	-
HVZ39_RS22725	4679531..4679728	+	198	WP_000839269.1	DUF957 domain-containing protein	-
HVZ39_RS22730	4679813..4680655	+	843	WP_001548928.1	DUF4942 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T164871 WP_000854726.1 NZ_CP057147:4678657-4679034 [Citrobacter sp. RHB35-C21]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T164871 NZ_CP073360:c4395408-4395235 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT164871 WP_001548930.1 NZ_CP057147:4678241-4678567 [Citrobacter sp. RHB35-C21]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT164871 NZ_CP073360:c4395732-4395436 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7