164870

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4437976..4438552 Replicon chromosome
Accession NZ_CP057147
Organism Citrobacter sp. RHB35-C21

Toxin (Protein)


Gene name shpA Uniprot ID A0A7L6U8Z2
Locus tag HVZ39_RS21515 Protein ID WP_049040418.1
Coordinates 4438265..4438552 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A7L6U766
Locus tag HVZ39_RS21510 Protein ID WP_046275900.1
Coordinates 4437976..4438278 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ39_RS21495 4434471..4434665 + 195 WP_003830038.1 YbdD/YjiX family protein -
HVZ39_RS21500 4434678..4435634 + 957 WP_115191028.1 GTPase -
HVZ39_RS21505 4435821..4437668 + 1848 WP_181632943.1 NERD domain-containing protein/DEAD/DEAH box helicase -
HVZ39_RS21510 4437976..4438278 - 303 WP_046275900.1 BrnA antitoxin family protein Antitoxin
HVZ39_RS21515 4438265..4438552 - 288 WP_049040418.1 BrnT family toxin Toxin
HVZ39_RS21520 4438786..4439952 + 1167 WP_019078303.1 restriction endonuclease -
HVZ39_RS21525 4440015..4440155 - 141 WP_165500049.1 hypothetical protein -
HVZ39_RS21530 4440118..4440416 + 299 Protein_4220 type I addiction module toxin, SymE family -
HVZ39_RS21535 4440562..4441053 - 492 Protein_4221 type VI secretion system tube protein Hcp -
HVZ39_RS21540 4441054..4441560 - 507 WP_046275904.1 hypothetical protein -
HVZ39_RS21545 4441560..4441922 - 363 WP_181633716.1 DUF2778 domain-containing protein -
HVZ39_RS21550 4442197..4443261 - 1065 WP_049041982.1 DUF2955 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(46-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11293.68 Da        Isoelectric Point: 7.5237

>T164870 WP_049040418.1 NZ_CP057147:c4438552-4438265 [Citrobacter sp. RHB35-C21]
MPMEFEWDANKAQSNHRKHGVRFEDAILVFDDPQHLSRQDRYENGEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERSRYEHS

Download         Length: 288 bp

>T164870 NZ_CP073360:c4297792-4297442 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11503.10 Da        Isoelectric Point: 9.5650

>AT164870 WP_046275900.1 NZ_CP057147:c4438278-4437976 [Citrobacter sp. RHB35-C21]
MNIVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDELWSEAHRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp

>AT164870 NZ_CP073360:c4298037-4297786 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCTTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6U8Z2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6U766

References