Detailed information of TA system TA164860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2618868..2619504
• Replicon: chromosome
• Accession: NZ_CP057147
• Organism: Citrobacter sp. RHB35-C21

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: HVZ39_RS12790
• Protein ID: WP_049259794.1
• Coordinates: 2618868..2619056 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HVZ39_RS12795
• Protein ID: WP_131382557.1
• Coordinates: 2619088..2619504 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ39_RS12770	2615646..2615876	-	231	WP_016153158.1	DUF2554 family protein	-
HVZ39_RS12775	2616066..2616191	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
HVZ39_RS12780	2616191..2617201	-	1011	WP_016153157.1	cytochrome d ubiquinol oxidase subunit II	-
HVZ39_RS12785	2617201..2618604	-	1404	WP_181632497.1	cytochrome ubiquinol oxidase subunit I	-
HVZ39_RS12790	2618868..2619056	+	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
HVZ39_RS12795	2619088..2619504	+	417	WP_131382557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HVZ39_RS12800	2619597..2621006	+	1410	WP_019076828.1	PLP-dependent aminotransferase family protein	-
HVZ39_RS12805	2621336..2622481	+	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
HVZ39_RS12810	2622498..2623514	+	1017	WP_016156582.1	ABC transporter ATP-binding protein	-
HVZ39_RS12815	2623515..2624459	+	945	WP_016153152.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T164860 WP_049259794.1 NZ_CP057147:2618868-2619056 [Citrobacter sp. RHB35-C21]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T164860 NZ_CP073360:1359881-1360279 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15064.33 Da
• Isoelectric Point: 4.7119

>AT164860 WP_131382557.1 NZ_CP057147:2619088-2619504 [Citrobacter sp. RHB35-C21]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGTEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT164860 NZ_CP073360:1359654-1359881 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9