Detailed information of TA system TA164856

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 670284..670963
• Replicon: chromosome
• Accession: NZ_CP057147
• Organism: Citrobacter sp. RHB35-C21

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: R8WLP2
• Locus tag: HVZ39_RS03320
• Protein ID: WP_006812012.1
• Coordinates: 670622..670963 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: R8WMM2
• Locus tag: HVZ39_RS03315
• Protein ID: WP_006812013.1
• Coordinates: 670284..670601 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ39_RS03270	665725..666546	+	822	WP_001548934.1	DUF945 domain-containing protein	-
HVZ39_RS03275	666763..667464	+	702	WP_001548933.1	WYL domain-containing protein	-
HVZ39_RS03280	667501..667737	+	237	WP_001115854.1	hypothetical protein	-
HVZ39_RS03285	667737..668177	+	441	WP_103468661.1	hypothetical protein	-
HVZ39_RS03290	668203..668670	+	468	WP_032200803.1	hypothetical protein	-
HVZ39_RS03295	668747..668986	+	240	WP_000194654.1	DUF905 domain-containing protein	-
HVZ39_RS03300	669084..669542	+	459	WP_000211838.1	antirestriction protein	-
HVZ39_RS03305	669558..670034	+	477	WP_000811693.1	RadC family protein	-
HVZ39_RS03310	670043..670264	+	222	WP_001548171.1	DUF987 domain-containing protein	-
HVZ39_RS03315	670284..670601	+	318	WP_006812013.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HVZ39_RS03320	670622..670963	+	342	WP_006812012.1	TA system toxin CbtA family protein	Toxin
HVZ39_RS03330	671326..671832	+	507	WP_181633221.1	G/U mismatch-specific DNA glycosylase	-
HVZ39_RS03335	671878..673722	-	1845	WP_016154549.1	RNA polymerase sigma factor RpoD	-
HVZ39_RS03340	673887..675632	-	1746	WP_181633222.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12989.07 Da
• Isoelectric Point: 10.4476

>T164856 WP_006812012.1 NZ_CP057147:670622-670963 [Citrobacter sp. RHB35-C21]
MKTLPATTQRAAKPCLAPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T164856 NZ_CP073360:c596349-595885 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11874.45 Da
• Isoelectric Point: 7.0166

>AT164856 WP_006812013.1 NZ_CP057147:670284-670601 [Citrobacter sp. RHB35-C21]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT164856 NZ_CP073360:c596684-596349 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: R8WLP2

Antitoxin

• AlphaFold DB: R8WMM2