Detailed information of TA system TA164839

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1036825..1037531
• Replicon: chromosome
• Accession: NZ_CP057136
• Organism: Citrobacter freundii strain RHB36-C06

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HVZ46_RS04900
• Protein ID: WP_181618724.1
• Coordinates: 1037163..1037531 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HVZ46_RS04895
• Protein ID: WP_181618723.1
• Coordinates: 1036825..1037142 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ46_RS04855	1032217..1032642	-	426	WP_105529709.1	glutaredoxin-dependent arsenate reductase	-
HVZ46_RS04860	1032655..1033944	-	1290	WP_105529710.1	arsenic transporter	-
HVZ46_RS04865	1033989..1034309	-	321	WP_105529711.1	transcriptional regulator	-
HVZ46_RS04870	1034395..1035093	+	699	WP_075146256.1	arsenical resistance protein ArsH	-
HVZ46_RS04875	1035254..1035525	+	272	Protein_951	DUF905 family protein	-
HVZ46_RS04880	1035628..1036086	+	459	WP_181618720.1	antirestriction protein	-
HVZ46_RS04885	1036095..1036577	+	483	WP_181618721.1	DNA repair protein RadC	-
HVZ46_RS04890	1036586..1036807	+	222	WP_181618722.1	DUF987 domain-containing protein	-
HVZ46_RS04895	1036825..1037142	+	318	WP_181618723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HVZ46_RS04900	1037163..1037531	+	369	WP_181618724.1	TA system toxin CbtA family protein	Toxin
HVZ46_RS04905	1037940..1038476	-	537	WP_135912505.1	tetratricopeptide repeat protein	-
HVZ46_RS04910	1038719..1039831	-	1113	WP_181618725.1	agmatine deiminase family protein	-
HVZ46_RS04915	1040231..1040794	+	564	WP_181618726.1	DJ-1/PfpI family protein	-
HVZ46_RS04920	1040995..1041978	+	984	WP_181618727.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1019947..1037531	17584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13714.93 Da
• Isoelectric Point: 8.5077

>T164839 WP_181618724.1 NZ_CP057136:1037163-1037531 [Citrobacter freundii]
MKTLPATISRAAKPCLSPVAVWQMLLTRLLEKHYGLSLNDTPFCDQSVIQEHIDAGITLANAVNFLVEKNELIRIDRNGF
TLQERTPYLTVTDILHAKRACGLMNGCSYREVSNIVLSRSRQ

Nucleotide

• Length: 369 bp

>T164839 NZ_CP073351:c5386339-5385956 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11956.60 Da
• Isoelectric Point: 7.3231

>AT164839 WP_181618723.1 NZ_CP057136:1036825-1037142 [Citrobacter freundii]
MNNTTWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECLKLDIAFPHFIRQMESMLTTGEMNPRHAHCVTLYH
NGFTCEADTFGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT164839 NZ_CP073351:c5386581-5386339 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA