Detailed information of TA system TA164832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4667044..4667660
• Replicon: chromosome
• Accession: NZ_CP057125
• Organism: Citrobacter sp. RHB36-C18

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HVZ52_RS22065
• Protein ID: WP_016155182.1
• Coordinates: 4667044..4667418 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: R8WIS4
• Locus tag: HVZ52_RS22070
• Protein ID: WP_016155181.1
• Coordinates: 4667418..4667660 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ52_RS22050	4664547..4665449	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
HVZ52_RS22055	4665446..4666081	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HVZ52_RS22060	4666078..4667007	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HVZ52_RS22065	4667044..4667418	-	375	WP_016155182.1	type II toxin-antitoxin system VapC family toxin	Toxin
HVZ52_RS22070	4667418..4667660	-	243	WP_016155181.1	ribbon-helix-helix domain-containing protein	Antitoxin
HVZ52_RS22075	4667865..4668773	+	909	WP_181598701.1	alpha/beta hydrolase	-
HVZ52_RS22080	4668838..4669080	+	243	WP_016155179.1	type II toxin-antitoxin system ParD family antitoxin	-
HVZ52_RS22085	4669073..4669360	+	288	WP_181598702.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HVZ52_RS22090	4669366..4670307	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HVZ52_RS22095	4670352..4670789	-	438	WP_149382140.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HVZ52_RS22100	4670786..4671658	-	873	WP_019077938.1	virulence factor BrkB family protein	-
HVZ52_RS22105	4671652..4672251	-	600	WP_016155174.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13678.84 Da
• Isoelectric Point: 8.5373

>T164832 WP_016155182.1 NZ_CP057125:c4667418-4667044 [Citrobacter sp. RHB36-C18]
MVTGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQESRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T164832 NZ_CP073351:873549-873956 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.72 Da
• Isoelectric Point: 4.3331

>AT164832 WP_016155181.1 NZ_CP057125:c4667660-4667418 [Citrobacter sp. RHB36-C18]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Nucleotide

• Length: 243 bp

>AT164832 NZ_CP073351:873302-873568 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: R8WIS4