Detailed information of TA system TA164830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2857894..2858484
• Replicon: chromosome
• Accession: NZ_CP057125
• Organism: Citrobacter sp. RHB36-C18

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A8I0G229
• Locus tag: HVZ52_RS13725
• Protein ID: WP_049270508.1
• Coordinates: 2857894..2858226 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A8I0KQR6
• Locus tag: HVZ52_RS13730
• Protein ID: WP_049270509.1
• Coordinates: 2858227..2858484 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ52_RS13700	2852931..2853290	-	360	WP_181598338.1	purine nucleoside phosphoramidase	-
HVZ52_RS13705	2853638..2855824	+	2187	WP_181598339.1	ferric-rhodotorulic acid/ferric-coprogen receptor FhuE	-
HVZ52_RS13715	2856003..2857646	+	1644	WP_181598340.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HVZ52_RS13725	2857894..2858226	-	333	WP_049270508.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HVZ52_RS13730	2858227..2858484	-	258	WP_049270509.1	antitoxin	Antitoxin
HVZ52_RS13735	2858815..2859000	-	186	Protein_2695	sigma-70 family RNA polymerase sigma factor	-
HVZ52_RS13740	2859113..2860087	-	975	WP_137351926.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11784.58 Da
• Isoelectric Point: 10.2965

>T164830 WP_049270508.1 NZ_CP057125:c2858226-2857894 [Citrobacter sp. RHB36-C18]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGVGTKTTGVIRCDQPRTI
DMGARNGKRLERIPEAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T164830 NZ_CP073351:354089-354436 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9384.88 Da
• Isoelectric Point: 4.4620

>AT164830 WP_049270509.1 NZ_CP057125:c2858484-2858227 [Citrobacter sp. RHB36-C18]
MYTTRLKKVGGSVMLAVPPAVLKTLALSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEEREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT164830 NZ_CP073351:354436-354735 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA