Detailed information of TA system TA164820

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 838522..839176
• Replicon: chromosome
• Accession: NZ_CP057125
• Organism: Citrobacter sp. RHB36-C18

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: HVZ52_RS04070
• Protein ID: WP_016154348.1
• Coordinates: 838769..839176 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: HVZ52_RS04065
• Protein ID: WP_016154349.1
• Coordinates: 838522..838788 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ52_RS04040	833735..835168	-	1434	WP_016154353.1	6-phospho-beta-glucosidase BglA	-
HVZ52_RS04045	835289..836017	-	729	WP_008785744.1	MurR/RpiR family transcriptional regulator	-
HVZ52_RS04050	836070..836381	+	312	WP_181598894.1	N(4)-acetylcytidine aminohydrolase	-
HVZ52_RS04055	836545..837204	+	660	WP_016154351.1	hemolysin III family protein	-
HVZ52_RS04060	837284..838264	-	981	WP_137352061.1	tRNA-modifying protein YgfZ	-
HVZ52_RS04065	838522..838788	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
HVZ52_RS04070	838769..839176	+	408	WP_016154348.1	protein YgfX	Toxin
HVZ52_RS04075	839277..839798	-	522	WP_016154347.1	flavodoxin FldB	-
HVZ52_RS04080	839912..840808	+	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
HVZ52_RS04085	840832..841545	+	714	WP_016154345.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HVZ52_RS04090	841551..843284	+	1734	WP_016157413.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T164820 WP_016154348.1 NZ_CP057125:838769-839176 [Citrobacter sp. RHB36-C18]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T164820 NZ_CP073317:c2244242-2243859 [Lacticaseibacillus rhamnosus]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAATAACGTTGGCAATCACTATAGTCCCACCGTCATTGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACCCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
GGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT164820 WP_016154349.1 NZ_CP057125:838522-838788 [Citrobacter sp. RHB36-C18]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT164820 NZ_CP073317:c2244510-2244262 [Lacticaseibacillus rhamnosus]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGCGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAATTGATTT
CTGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6