Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 27595..28196 Replicon plasmid pRHB38-C01_2
Accession NZ_CP057105
Organism Escherichia fergusonii strain RHB38-C01

Toxin (Protein)


Gene name doc Uniprot ID F0JYA5
Locus tag HVZ66_RS23065 Protein ID WP_001216044.1
Coordinates 27595..27975 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag HVZ66_RS23070 Protein ID WP_001190712.1
Coordinates 27975..28196 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ66_RS23040 23035..24519 - 1485 WP_000124159.1 hypothetical protein -
HVZ66_RS23045 24519..25712 - 1194 WP_000219619.1 hypothetical protein -
HVZ66_RS23050 25799..26251 - 453 WP_001312282.1 late promoter-activating protein (Gp10) -
HVZ66_RS23055 26340..27383 - 1044 WP_000648835.1 DUF968 domain-containing protein -
HVZ66_RS23060 27411..27590 - 180 WP_000113018.1 hypothetical protein -
HVZ66_RS23065 27595..27975 - 381 WP_001216044.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HVZ66_RS23070 27975..28196 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HVZ66_RS23075 28582..29562 - 981 WP_000019434.1 IS5-like element ISKpn26 family transposase -
HVZ66_RS23080 29825..31213 + 1389 WP_001207923.1 nitrate/nitrite transporter NarU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..97856 97856
- flank IS/Tn - - 28582..29562 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13544.28 Da        Isoelectric Point: 5.6343

>T164818 WP_001216044.1 NZ_CP057105:c27975-27595 [Escherichia fergusonii]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Download         Length: 381 bp

>T164818 NZ_CP073315:c1624592-1624242 [Psychrobacillus sp. INOP01]
TTGATCGTAAAACGTGGAGACGTTTTTTTTGCGGATTTATCACCAGTAATCGGGTCTGAGCAAGGTGGAACCAGACCCGT
TTTGGTGATACAAAATGATATCGGTAATAGATTCAGTCCAACTGTCATTGTGGCAGCTATCACAGCTCAAATTCAAAAGG
CAAAATTACCAACGCATGTAGAGATAAATGCAAAAGAATATGATTTTGAACGTGATTCGGTTATTTTATTGGAACAGTTA
AGAACAATTGATAAATCTCGACTCACGGATAAAATCACCCACCTAGACGTAGACGTGATGGAAAATGTGGATCATGCATT
AGGGATTAGCTTAGGGATCGTTAAATTTTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT164818 WP_001190712.1 NZ_CP057105:c28196-27975 [Escherichia fergusonii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT164818 NZ_CP073315:c1624878-1624597 [Psychrobacillus sp. INOP01]
GTGGTAAGTGATAAAAAGAAAAAGGAAGCGATTATTCAGCTTCCACATCAATTGGTTAATGAAGGAGTAAAAACCGTAAA
ACAATCATTAACTGGCGTAGAAAGTTTCGTTCGTGTCCAAGCAAATGAATATATAAAAAATGGTCAGGCAAATCTTATAA
GAGAAGCGATGATGAAGGGCTATGTTGAGATGTCTCATATAAATCTATCCATTTGTACGGAGTGTTTACATGCAGAATAC
GAAGCTCAACATACAACAGAGCGACTCGTAAGTGGAGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6Y6HMN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References