Detailed information of TA system TA164811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4209856..4210656
• Replicon: chromosome
• Accession: NZ_CP057104
• Organism: Escherichia fergusonii strain RHB38-C01

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A891SNC1
• Locus tag: HVZ66_RS20480
• Protein ID: WP_000342448.1
• Coordinates: 4209856..4210383 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: HVZ66_RS20485
• Protein ID: WP_181590918.1
• Coordinates: 4210390..4210656 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ66_RS20455	4204931..4205698	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
HVZ66_RS20460	4205695..4206972	-	1278	WP_000803807.1	branched chain amino acid ABC transporter permease LivM	-
HVZ66_RS20465	4206969..4207895	-	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HVZ66_RS20470	4207943..4209052	-	1110	WP_181590916.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
HVZ66_RS20475	4209476..4209859	+	384	WP_181590917.1	aspartate 1-decarboxylase autocleavage activator PanM	-
HVZ66_RS20480	4209856..4210383	-	528	WP_000342448.1	GNAT family N-acetyltransferase	Toxin
HVZ66_RS20485	4210390..4210656	-	267	WP_181590918.1	DUF1778 domain-containing protein	Antitoxin
HVZ66_RS20490	4210806..4211909	-	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
HVZ66_RS20495	4212181..4213035	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
HVZ66_RS20500	4213280..4214338	-	1059	WP_001042003.1	permease-like cell division protein FtsX	-
HVZ66_RS20505	4214331..4214999	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19692.68 Da
• Isoelectric Point: 7.3232

>T164811 WP_000342448.1 NZ_CP057104:c4210383-4209856 [Escherichia fergusonii]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T164811 NZ_CP073310:3355605-3355976 [Enterobacter cloacae]
GTGAATAATATGGCCGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTCGAAGCTGCGGATGCGATTGA
TCATGCCGCGTCACATCGAGCCATTAGCTTAATTACCTGGATGGAGGTGATGGTGGGCGCACGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTCGAAATTATTGATGTGTCTCGGGATATCGCGGAAAGAAGCGTTTTACTGCGT
GAAAAGCATGGAATGAAACTGCCTGATGCCATCATTCTGGCCACAGCCCAGAGCAGAAAATGTCCGTTGATTTCTCGAAA
TACGAAAGATTTTTCGGGTATTGATGAGGTCGTTACGCCTTATCAACTGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9954.41 Da
• Isoelectric Point: 10.0863

>AT164811 WP_181590918.1 NZ_CP057104:c4210656-4210390 [Escherichia fergusonii]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNLPSPGEKLKR
AVKRLQGM

Nucleotide

• Length: 267 bp

>AT164811 NZ_CP073310:3355360-3355602 [Enterobacter cloacae]
ATGAGTGTTTTGGCGGAACTGGATATGGGCAGAATACTGATCGATTTACCTGATGACGTGATTCAACGGCTGGATAACCT
CAAGAAACTGCGAAATCAGCCGCGCGCTGAGCTGCTACGGGAGGCGATAGAGCTGTATCTCGACCAGCAGAGTTCGTCTG
TTATTCGCGCTGCGCTTGGCTTGTGGGGAGACAAGAAAGAAGACGGACTGGAGTATGAACGTAAGCTGCGTGAGGAATGG
TGA

Structures

Toxin

• PDB: 6GTS