Detailed information of TA system TA164787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 654375..654925
• Replicon: chromosome
• Accession: NZ_CP057104
• Organism: Escherichia fergusonii strain RHB38-C01

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HVZ66_RS03310
• Protein ID: WP_181591135.1
• Coordinates: 654611..654925 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: HVZ66_RS03305
• Protein ID: WP_000125566.1
• Coordinates: 654375..654608 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ66_RS03280	649447..649734	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
HVZ66_RS03285	649793..651124	+	1332	WP_001183184.1	MFS transporter	-
HVZ66_RS03290	651232..651762	+	531	WP_032183187.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
HVZ66_RS03295	651755..653617	+	1863	WP_181591134.1	glutathione-regulated potassium-efflux system protein KefC	-
HVZ66_RS03300	653810..654289	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
HVZ66_RS03305	654375..654608	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HVZ66_RS03310	654611..654925	+	315	WP_181591135.1	CcdB family protein	Toxin
HVZ66_RS03315	654922..655770	-	849	WP_000257193.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
HVZ66_RS03320	655777..656154	-	378	WP_063101160.1	Co2+/Mg2+ efflux protein ApaG	-
HVZ66_RS03325	656157..656978	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
HVZ66_RS03330	656975..657964	-	990	WP_000241252.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
HVZ66_RS03335	657964..659250	-	1287	WP_000800490.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11736.73 Da
• Isoelectric Point: 8.0666

>T164787 WP_181591135.1 NZ_CP057104:654611-654925 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLEGI

Nucleotide

• Length: 315 bp

>T164787 NZ_CP073285:c4756514-4755981 [Klebsiella pneumoniae subsp. ozaenae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT164787 WP_000125566.1 NZ_CP057104:654375-654608 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT164787 NZ_CP073285:c4756791-4756525 [Klebsiella pneumoniae subsp. ozaenae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9