Detailed information of TA system TA164765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 65357..66093
• Replicon: plasmid pRHB38-C06_2
• Accession: NZ_CP057100
• Organism: Klebsiella pneumoniae strain RHB38-C06

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HVZ68_RS25895
• Protein ID: WP_077269838.1
• Coordinates: 65611..66093 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HVZ68_RS25890
• Protein ID: WP_003026799.1
• Coordinates: 65357..65623 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ68_RS25865	61453..61716	+	264	WP_009310051.1	hypothetical protein	-
HVZ68_RS25870	61713..62279	+	567	WP_009654312.1	hypothetical protein	-
HVZ68_RS25875	62310..62804	+	495	WP_004213594.1	hypothetical protein	-
HVZ68_RS25880	62865..63068	+	204	WP_004213596.1	hemolysin expression modulator Hha	-
HVZ68_RS25885	63778..64695	-	918	WP_069985721.1	bifunctional helix-turn-helix transcriptional regulator/GNAT family N-acetyltransferase	-
HVZ68_RS25890	65357..65623	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HVZ68_RS25895	65611..66093	+	483	WP_077269838.1	GNAT family N-acetyltransferase	Toxin
HVZ68_RS25900	66304..67650	+	1347	WP_077251107.1	ISNCY family transposase	-
HVZ68_RS25905	67810..68514	+	705	WP_040167821.1	toll/interleukin-1 receptor domain-containing protein	-
HVZ68_RS25910	68922..69293	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
HVZ68_RS25915	69310..69510	-	201	Protein_65	acireductone synthase	-
HVZ68_RS25920	69507..70121	-	615	WP_119127151.1	methylthioribulose 1-phosphate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iutA / iucD / iucC / iucB / iucA	1..140023	140023
	-	flank	IS/Tn	-	-	66292..67650	1358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17378.94 Da
• Isoelectric Point: 9.6047

>T164765 WP_077269838.1 NZ_CP057100:65611-66093 [Klebsiella pneumoniae]
VGRITAPEPLSSSHQRAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T164765 NZ_CP073262:c3061030-3060710 [Duffyella gerundensis]
ATGCAAATTTCATCTGTACCGGCCACGGTGCCGGTTTCGTCGCGCCTGTCGCCCGTGCAGGTGTGGCAGCAATTGTTAAC
CTATCTGCTGGAACACCATTACGGCCTCACACTCAACGACACACCATTCCATGATGACGCGACCATTGAGGAGCATATCG
AAGCGGGAATAACGCTTGCCGATGCTGTTAATTTCCTGGTGGAACGTTATGAACTGGTACGCATCGACCGTAAAGGATTT
ACATGGCAAGAGCAGACACCGTTCCTGACCGCCACCGATATTCTCAGAGCTAGGCGAGCTACCGGATTAATAAATACTTG
A

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT164765 WP_003026799.1 NZ_CP057100:65357-65623 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT164765 NZ_CP073262:c3061386-3061063 [Duffyella gerundensis]
ATGGCGTTATCTGTCTCTCAGGAATGGGGCCTGCGAAGTAATATCACACCACGTTTTGGCGCAAGATTGGTTCAGGAAGG
CAACCGCCTGCATTACCTGGCTGACAGGGCTGAGTTGACGGGGAGCTTTACCCCGGAGCAATTACAGAAACTGGATCGGG
CATTCCCGCTATTTATAGAACAACTGGAAAATTCGCTGCGCTCTGGTGAGTTAAATCCATGTCAGCAACATCGGGTAGGA
GTCCATATTGCCGGGTTGACCTGCATAGCAGATACCCTGGGCAGTTACGGCTATGTTTACATTTCTATTTACCCAACCCA
GTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66