Detailed information of TA system TA164764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 56613..57256
• Replicon: plasmid pRHB38-C06_2
• Accession: NZ_CP057100
• Organism: Klebsiella pneumoniae strain RHB38-C06

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HVZ68_RS25855
• Protein ID: WP_069985717.1
• Coordinates: 56840..57256 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6B7Q5S4
• Locus tag: HVZ68_RS25850
• Protein ID: WP_021312476.1
• Coordinates: 56613..56843 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ68_RS25825	52192..52713	-	522	WP_032448294.1	hypothetical protein	-
HVZ68_RS25830	52771..53163	-	393	WP_074422814.1	hypothetical protein	-
HVZ68_RS25835	53272..54216	-	945	WP_181590012.1	hypothetical protein	-
HVZ68_RS25840	54762..55451	+	690	WP_157263246.1	hypothetical protein	-
HVZ68_RS25845	55483..56172	-	690	WP_069985716.1	RES family NAD+ phosphorylase	-
HVZ68_RS25850	56613..56843	+	231	WP_021312476.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HVZ68_RS25855	56840..57256	+	417	WP_069985717.1	type II toxin-antitoxin system VapC family toxin	Toxin
HVZ68_RS25860	57302..61105	-	3804	WP_181590013.1	pcar	-
HVZ68_RS25865	61453..61716	+	264	WP_009310051.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iutA / iucD / iucC / iucB / iucA	1..140023	140023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14989.47 Da
• Isoelectric Point: 9.2738

>T164764 WP_069985717.1 NZ_CP057100:56840-57256 [Klebsiella pneumoniae]
VKKTFMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T164764 NZ_CP073262:2633424-2633642 [Duffyella gerundensis]
ATGAGTGACAAAACACTGACTAAAACTGATTACCTGATGCGCTTAAGACGTTGCCGATCTATCGATACCCTTGAGCGTGT
TATTGAAAAAAACAAATATGAATTACCCGATAATGAACTGGCCATATTTTATTCAGCTGCCGATCATCGTTTAGCAGAAC
TGACGATGAATAAGCTTTACGATAAAGTGCCGGTTTCTGTCTGGAAATTTGTACGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8723.76 Da
• Isoelectric Point: 4.3410

>AT164764 WP_021312476.1 NZ_CP057100:56613-56843 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT164764 NZ_CP073262:2633017-2633391 [Duffyella gerundensis]
ATGGACGAATACTCACCTAAAAGGCATGATATGGCGCAGCTTAAGTACCTGTGCGAAAGTCTGCATGACGATAGCATGGC
CACCTTAAGTGACAGCCATCACGGCTGGGTTAATGATCCAACCTCTGCCAGCAATTTGCAGCTCAATGACCTGATTGAAC
ATATCGCATCCTTCACCATGAATTACAAAATTAAACATGTTGAAGATGAAGAACTGATTACTCAGATTGATGAATATCTT
GACGATACTTTTATGTTATTTAGTAACTATGGCGTAAGTGCGCAAGATCTGCAGCGTTGGCAACGTTCGGCAAAGCGGCT
TTTTAATATATTTGCCGAGGAATGCGCTCTGTTACAACCGAGCCATTCATTTTAG

Structures

Antitoxin

• AlphaFold DB: A0A6B7Q5S4