Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5153409..5154034 Replicon chromosome
Accession NZ_CP057099
Organism Klebsiella pneumoniae strain RHB38-C06

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HVZ68_RS25225 Protein ID WP_002882817.1
Coordinates 5153409..5153792 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HVZ68_RS25230 Protein ID WP_004150355.1
Coordinates 5153792..5154034 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ68_RS25210 5150775..5151677 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HVZ68_RS25215 5151674..5152309 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HVZ68_RS25220 5152306..5153235 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HVZ68_RS25225 5153409..5153792 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HVZ68_RS25230 5153792..5154034 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HVZ68_RS25235 5154239..5155156 + 918 WP_065803683.1 alpha/beta hydrolase -
HVZ68_RS25240 5155170..5156111 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HVZ68_RS25245 5156156..5156593 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HVZ68_RS25250 5156590..5157450 - 861 WP_004146232.1 virulence factor BrkB family protein -
HVZ68_RS25255 5157444..5158043 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T164763 WP_002882817.1 NZ_CP057099:c5153792-5153409 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T164763 NZ_CP073262:c1800883-1800413 [Duffyella gerundensis]
GTGAAGTTGTACCGCCTGACCAAAACACGCTATCTGAGTTCTGCCTGGAGCGGTTACGGTGCCAGAGAAGGCGGCGGGCG
CTGGAATAATGTAGGGGTGTCGATGGTCTATGCATCGGAAACCGCGTCGCTGACCATGCTGGAAATACTGGTGCATCTGC
AAAGTGCCGCCACGCTGGATGCTTATACCTTGCTTTGTGTTGAGGTGCCCGATGAGTTGATTCAGTGGATTGATGTGACG
CAGTTACCGGAAAACTGGCGGGCGGCCGAAGCCCCGGCGGCGCTGCGTGAGATTGGTGATGCCTGGATCAGCAGCGGCGA
GTCGGTGGCGCTGCGCATACCCAGTGCGCTGTCGCCGGTGGAACATAACTTTTTGCTTAATCCGGATCATCCCGCCTTCA
CGGAGCTGGTCAGCCGCGCCAGTCATATTCCGTTTATGTTTGATTCACGCTTTGGTACGGCGCATTCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT164763 WP_004150355.1 NZ_CP057099:c5154034-5153792 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT164763 NZ_CP073262:c1801323-1800880 [Duffyella gerundensis]
ATGAAACAGTTTACCTTACCTGCACGGCATCACTCGCCAGAACGCCTCTGGCAGGTTGCCGGGCTGAACAGCGCTGACGG
CGTGATGCTAATGGGGCAGATCGATCGCGGGCTGGAAGGCGCGGTGGCCAACCGTATTATTTCGTGGGCGCTGATGACCC
AGGCCGATTTGCGACACGTGACCGGCATTCCTGCCACCACCTTTAACCGCGGCATGAAAGATCGCTTTACCGCCGAGCAG
AGCGAGCGCCTGGTGCGCTTTATTCGCGTGATGGATCGCGCCGTTGAGCTGTTTGAAGGCGATAAGCATAAGGCGCAGCA
GTGGCTGAATGAGCCGGCAAGGGCGCTTGATAACAAAAAGCCTGCCGACCTGGTTTCATCGGAAACCGGCGCCTATGAGG
TACTAAAACTGATTACCCGGCTTGAGCACGGTGTTTATTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References