Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4559567..4560377 Replicon chromosome
Accession NZ_CP057099
Organism Klebsiella pneumoniae strain RHB38-C06

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HVZ68_RS22455 Protein ID WP_004178461.1
Coordinates 4559567..4560100 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HVZ68_RS22460 Protein ID WP_002887278.1
Coordinates 4560111..4560377 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ68_RS22450 4558398..4559519 + 1122 WP_004214138.1 cupin domain-containing protein -
HVZ68_RS22455 4559567..4560100 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
HVZ68_RS22460 4560111..4560377 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
HVZ68_RS22465 4560480..4561913 - 1434 WP_023283180.1 Cu(+)/Ag(+) sensor histidine kinase -
HVZ68_RS22470 4561903..4562586 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
HVZ68_RS22475 4562758..4564143 + 1386 WP_023283179.1 efflux transporter outer membrane subunit -
HVZ68_RS22480 4564161..4564505 + 345 WP_021462623.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T164760 WP_004178461.1 NZ_CP057099:c4560100-4559567 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T164760 NZ_CP073248:c2047905-2047519 [Proteus mirabilis]
ATGGTTATTTATAAAACAAAGATGTTCAAAAATAGTTTCAAAAAAATAACGCTTACGAATGCAGAACTAATAGCTATCGC
TACCGAAGTCCTTGCAGGACAATTTGAGGCAGATCTAGGTGGTGGTGTTATCAAGAAACGTGCCTGTATCCAAGGAAAAG
GTAAAAGTAGCGGAATTAGAACAATTATATTTTATAAGCAAGGGAATAATTTATTCTTTGCTGATGGTTGGAAAAAATCG
AGTTTAAGCTCTAAAAAAACCAAAGAGATCACAGATGATGAACTTGAGTCCTATAAAGATCTAGCAAAAGATCTCTTCAA
TGCAAATCAAAATAAAATAGATAAAATGATTGCACTAGGCCTTTTAACAGAGGTTAAATATGACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT164760 WP_002887278.1 NZ_CP057099:c4560377-4560111 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT164760 NZ_CP073248:c2047526-2047206 [Proteus mirabilis]
ATGACTGATAAAATGAAAGAAATGCTAAAATTTGCACAAGAAATGGAAGATGCAGGAGTCACTGATGGCAGTACAACTAA
ACGTATTGCCGCCAGAATAAAAGCGAAAGAATTACAAGCTAAGTATAAACATCCTACCCCTATGACAGGTGAAGATATAA
AAAGGATCCGTGAGCAAAATAATATCTCTCAATCTTCTCTTGCTTTGATATTGAATATGACTAAAGAAAGCGTTTCTAAG
TGGGAAAGAAACGAAATTAAGCCCAATGGTGCAGCACTACGACTTCTGCATTTAATAGATAAAAAAATATTATTCATCTA
G

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References