Detailed information of TA system TA164747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/FR47-DUF1778
• Location: 40914..41712
• Replicon: plasmid pRHB38-C07_2
• Accession: NZ_CP057094
• Organism: Escherichia fergusonii strain RHB38-C07

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: HVZ69_RS23185
• Protein ID: WP_077580870.1
• Coordinates: 41191..41712 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: HVZ69_RS23180
• Protein ID: WP_001711191.1
• Coordinates: 40914..41183 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ69_RS23155	36341..37681	-	1341	WP_087904724.1	AAA family ATPase	-
HVZ69_RS23160	37742..38467	-	726	WP_097418314.1	hypothetical protein	-
HVZ69_RS23165	38750..39517	+	768	WP_001711193.1	hypothetical protein	-
HVZ69_RS23170	39570..39923	-	354	WP_161953766.1	hypothetical protein	-
HVZ69_RS23175	39929..40594	-	666	WP_023135658.1	AAA family ATPase	-
HVZ69_RS23180	40914..41183	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
HVZ69_RS23185	41191..41712	+	522	WP_077580870.1	GNAT family N-acetyltransferase	Toxin
HVZ69_RS23190	41881..42132	-	252	WP_023135660.1	hypothetical protein	-
HVZ69_RS23195	42135..42827	-	693	WP_024172707.1	membrane protein	-
HVZ69_RS23200	42841..43164	-	324	WP_077580871.1	hypothetical protein	-
HVZ69_RS23205	43298..43879	-	582	WP_077580872.1	tail fiber assembly protein	-
HVZ69_RS23210	43888..44791	-	904	Protein_46	macro domain-containing protein	-
HVZ69_RS23215	44788..45744	-	957	Protein_47	tail fiber protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106378	106378

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19428.29 Da
• Isoelectric Point: 8.6369

>T164747 WP_077580870.1 NZ_CP057094:41191-41712 [Escherichia fergusonii]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKRLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T164747 NZ_CP073244:c2770898-2770407 [Proteus terrae subsp. cibarius]
ATGGGAAAAGTAACAGCACCTGAGCCGTTATCAAACTCACATGAAGTGGCTGATTTTTATAGTAGTGAAAATGTATTAGA
TAACTGGATAAAACAAAGAGGTTTAAAAAACCAATTTTTAGGTGCATCCCGTACATTTGTGGTCTGTGAAAAAAATAAAA
AACGTGTTGTTGGGTACTATTCTTTAGCAACAGGTAGTGTTAACCATACTGAAGCAATGAGTCATATTCGTCGCAATATG
CCAGACCCAATACCCGTGATCATTTTAGCTAGACTTGCTGTTGATAAAACTTTTCATGGAAAAGCTCTTGGTGCCGATTT
GTTAAGAGATGCAGTTTTGCGTTGTCATCATGTAGCTGAAAACATTGGTGTTAGAGCGATTATGGTTCATGCTTTAACTG
AAAACGCAAAACATTTCTACCTTCATCATGGTTTCAAAGCTTCTTCGACTCAAGACAAAATCTTATTCTTAGCACTAAAA
AAGAAAATATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT164747 WP_001711191.1 NZ_CP057094:40914-41183 [Escherichia fergusonii]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT164747 NZ_CP073244:c2771152-2770886 [Proteus terrae subsp. cibarius]
ATGAAAGCTGATGTACAACTCAATATTCGCGCTAAAGAATCACAACGCGCACTTATTGATACTGCTGCTGAAATTCTCCA
TAAATCTCGTACTGACTTTATTCTTGAGACAGCATGCCAAGCTGCTGAAGACGTCATTTTAGACCGTAGAACCTTTAATT
TAAATGATGCTCAATATGCGGAATTTATAGAAATTATTGATGCGCCGGTTGATATTGACCCTGCTCTTGAAAAGTTATTA
ATGAGAAAACCATTATGGGAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2