Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4742784..4743594 Replicon chromosome
Accession NZ_CP057069
Organism Klebsiella pneumoniae strain RHB39-C09

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HVZ81_RS23260 Protein ID WP_004178461.1
Coordinates 4742784..4743317 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HVZ81_RS23265 Protein ID WP_002887278.1
Coordinates 4743328..4743594 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ81_RS23255 4741615..4742736 + 1122 WP_020804439.1 cupin domain-containing protein -
HVZ81_RS23260 4742784..4743317 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
HVZ81_RS23265 4743328..4743594 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
HVZ81_RS23270 4743697..4745130 - 1434 WP_020804438.1 Cu(+)/Ag(+) sensor histidine kinase -
HVZ81_RS23275 4745120..4745803 - 684 WP_020804437.1 copper response regulator transcription factor CusR -
HVZ81_RS23280 4745975..4747360 + 1386 WP_020804440.1 efflux transporter outer membrane subunit -
HVZ81_RS23285 4747378..4747722 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T164713 WP_004178461.1 NZ_CP057069:c4743317-4742784 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T164713 NZ_CP073105:c3856041-3855688 [Stutzerimonas stutzeri]
ATGACTTGGGACGTTGAGTACACCGATGAGTTTGGTGGCTGGTGGGAGTCACTCACTGCGGAGGAGCAGGAGTCCATAGC
GGTCTCCGTGCGTTTGCTGGAAGACCGTGGGCCTTCGCTCGGATTCCCACATAGCAGCGCTATCAATGGGGCACGTCATG
GGCATATGCGAGAGCTGAGAACCCAGCACAACGGGCGCCCGATACGAACGCTGTATGCATTCGATCCCAGGCGCTCGGCG
ATCCTGCTGATTGGCGGAGACAAGACGGGCGACAACCGCTGGTATGAAATCAACGTTCCGTTGGCAGATCGTCTCTATGA
CGAGCACCTGGAACAGCTTGAGAAGGAGGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT164713 WP_002887278.1 NZ_CP057069:c4743594-4743328 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT164713 NZ_CP073105:c3855684-3855355 [Stutzerimonas stutzeri]
ATGGCCAAGAAATTCGCAGACTTACGCGCAAAGATGTCGCCAGAGGCTCAGGCTCGCGTGGAAGCCAAAGCCCAGGAGCT
GCTTGCTGAAATGCCGCTGAACGAACTGCGCCAGGCACGTGGCTTGTCGCAGAAGATGCTGGCTGAGCTACTGCACGTAC
AGCAGCCGTCGATCGCGAAAATGGAAAAGCGCACCGACATGTATATCTCCACGCTGCGTAGCCACATCGAAGCAATGGGC
GGGCAGCTCGAGGTCATCGCCCGGTTCCCGGATGGTTCGGTGAAGATCAGCAACTTCGCCGAGATCGAAGCTGCCGCTCA
GCAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References