Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4481324..4482134 Replicon chromosome
Accession NZ_CP057066
Organism Klebsiella pneumoniae strain RHB39-C10

Toxin (Protein)


Gene name KacT Uniprot ID A0A6G4MHE5
Locus tag HVZ82_RS21900 Protein ID WP_065801222.1
Coordinates 4481324..4481857 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HVZ82_RS21905 Protein ID WP_002887278.1
Coordinates 4481868..4482134 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVZ82_RS21895 4480155..4481276 + 1122 WP_004214138.1 cupin domain-containing protein -
HVZ82_RS21900 4481324..4481857 - 534 WP_065801222.1 GNAT family N-acetyltransferase Toxin
HVZ82_RS21905 4481868..4482134 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
HVZ82_RS21910 4482237..4483670 - 1434 WP_065801221.1 Cu(+)/Ag(+) sensor histidine kinase -
HVZ82_RS21915 4483660..4484343 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
HVZ82_RS21920 4484515..4485900 + 1386 WP_009484676.1 efflux transporter outer membrane subunit -
HVZ82_RS21925 4485918..4486262 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19868.73 Da        Isoelectric Point: 5.2614

>T164687 WP_065801222.1 NZ_CP057066:c4481857-4481324 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRSYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKVFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T164687 NZ_CP073075:c4122498-4122238 [Gordonia polyisoprenivorans]
ATGTCTATCAGTGCGAGTGAGGCGCGGAAGTTGCTGTTTCCGCTGATCGAGCAGGTCAACGACGATCGGAGTGCGATCGA
AATCGTTTCTCGGAAGGGCAATGCGGTGCTGATGTCGGCCGACGAGTACGCGGCGTGGCAGGAGACGGCCTACCTGTTCA
GGTCGCCGGCCAACGCCCGCCGACTGTTGGAGTCCTACACACGTGTCTCCGTGGGGCAGACCGAGGTGCATGACCTCGAC
CGGACCGACGAGGACGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT164687 WP_002887278.1 NZ_CP057066:c4482134-4481868 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT164687 NZ_CP073075:c4122237-4121983 [Gordonia polyisoprenivorans]
GTGCGTCTGGTCTGGGACCAATCGGCATGGGAAGACTATGTGCACTGGCAAACCGCTGACCGGCGACTACTGAAGCGGAT
CAACACCCTGCTCGGCGCGTGCCTGCGCGATGCATTCACCGGCATCGGAAAACCCGAACCGCTCAAGTACGGAGTCCCGG
GCGCCTGGTCGCGGCGCATCTCCGACGAACACCGGCTCGTCTACCTCGTCGATGGCGATGACTTGATCATTCTGCAGGCT
CGGTACCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G4MHE5

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References