Detailed information of TA system TA164671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 108239..108975
• Replicon: plasmid pRHB39-C24_2
• Accession: NZ_CP057035
• Organism: Klebsiella pneumoniae strain RHB39-C24

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: HVZ90_RS25845
• Protein ID: WP_004098919.1
• Coordinates: 108239..108721 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: HVZ90_RS25850
• Protein ID: WP_032415032.1
• Coordinates: 108709..108975 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ90_RS25820	103476..104090	+	615	WP_045420631.1	DJ-1/PfpI family protein	-
HVZ90_RS25825	104102..104620	+	519	WP_038991646.1	GrpB family protein	-
HVZ90_RS25830	104664..106073	-	1410	WP_038991643.1	ISNCY family transposase	-
HVZ90_RS25835	106242..106637	-	396	WP_080863043.1	helix-turn-helix domain-containing protein	-
HVZ90_RS25840	106686..108032	-	1347	WP_080863044.1	ISNCY family transposase	-
HVZ90_RS25845	108239..108721	-	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
HVZ90_RS25850	108709..108975	-	267	WP_032415032.1	DUF1778 domain-containing protein	Antitoxin
HVZ90_RS25855	109151..109405	-	255	WP_044246670.1	hypothetical protein	-
HVZ90_RS25860	109526..109681	+	156	Protein_121	IS5/IS1182 family transposase	-
HVZ90_RS25865	109994..110392	-	399	WP_004181717.1	H-NS histone family protein	-
HVZ90_RS25870	110795..111613	-	819	WP_004181718.1	phospholipase D family protein	-
HVZ90_RS25875	111722..112216	-	495	WP_038992354.1	thermonuclease family protein	-
HVZ90_RS25880	112399..113670	+	1272	WP_004181719.1	DUF1173 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..227199	227199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T164671 WP_004098919.1 NZ_CP057035:c108721-108239 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T164671 NZ_CP073061:c57109-56807 [Acinetobacter baumannii]
ATGTACTCAATATATACCACAGAAGCCTTTGATGATTGGTTTACCAAGTTAAAAGATCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGGAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTTTCTGAGTTACGTTTCT
TCTTTGGACCTGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTCGTTATTCTCTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10123.53 Da
• Isoelectric Point: 4.5141

>AT164671 WP_032415032.1 NZ_CP057035:c108975-108709 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT164671 NZ_CP073061:c56806-56525 [Acinetobacter baumannii]
ATGGCTATCAAACTTCGTAAATGGGATAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCTGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAACAATTTAA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928