Detailed information of TA system TA164615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4184762..4185338
• Replicon: chromosome
• Accession: NZ_CP056967
• Organism: Citrobacter freundii strain RHB41-C16

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HV011_RS19795
• Protein ID: WP_181595920.1
• Coordinates: 4185051..4185338 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HV011_RS19790
• Protein ID: WP_181595919.1
• Coordinates: 4184762..4185064 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV011_RS19775	4181230..4183380	+	2151	WP_096758430.1	pyruvate/proton symporter BtsT	-
HV011_RS19780	4183488..4183682	+	195	WP_003830038.1	YbdD/YjiX family protein	-
HV011_RS19785	4183695..4184651	+	957	WP_096758431.1	GTPase	-
HV011_RS19790	4184762..4185064	-	303	WP_181595919.1	BrnA antitoxin family protein	Antitoxin
HV011_RS19795	4185051..4185338	-	288	WP_181595920.1	BrnT family toxin	Toxin
HV011_RS19800	4185511..4185675	-	165	WP_117342370.1	DUF1127 domain-containing protein	-
HV011_RS19805	4185852..4187264	+	1413	WP_181595921.1	PLP-dependent aminotransferase family protein	-
HV011_RS19810	4187291..4188253	-	963	WP_181595922.1	Rpn family recombination-promoting nuclease/putative transposase	-
HV011_RS19815	4188527..4189768	+	1242	WP_181595923.1	multidrug efflux MFS transporter MdtM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (42-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11334.69 Da
• Isoelectric Point: 7.5239

>T164615 WP_181595920.1 NZ_CP056967:c4185338-4185051 [Citrobacter freundii]
MPMEFEWDANKAQSNHRKHGVRFEDAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIVVILVAHSVRFESGFEAIRIIS
ARKADRKERNRYEHD

Nucleotide

• Length: 288 bp

>T164615 NZ_CP072998:c46903-46487 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11487.03 Da
• Isoelectric Point: 8.5366

>AT164615 WP_181595919.1 NZ_CP056967:c4185064-4184762 [Citrobacter freundii]
MSMIKHKRGSVSALSAQHEAELKALANKPDEDIDYSDIPVTEDEQWSEANRGTFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT164615 NZ_CP072998:c47130-46900 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA