Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 67421..68022 Replicon plasmid pRHB41-C20_3
Accession NZ_CP056963
Organism Escherichia fergusonii strain RHB41-C20

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag HV012_RS23625 Protein ID WP_001216045.1
Coordinates 67421..67801 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag HV012_RS23630 Protein ID WP_001190712.1
Coordinates 67801..68022 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV012_RS23600 62862..64346 - 1485 WP_000124150.1 terminase -
HV012_RS23605 64346..65539 - 1194 WP_000219625.1 hypothetical protein -
HV012_RS23610 65625..66077 - 453 WP_001326849.1 late promoter-activating protein -
HV012_RS23615 66166..67209 - 1044 WP_112075408.1 DUF968 domain-containing protein -
HV012_RS23620 67237..67416 - 180 WP_001339207.1 hypothetical protein -
HV012_RS23625 67421..67801 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HV012_RS23630 67801..68022 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HV012_RS23635 68095..68484 - 390 WP_000506730.1 DNA repair protein -
HV012_RS23640 68659..69243 + 585 WP_023356284.1 hypothetical protein -
HV012_RS23645 69244..69606 + 363 WP_042102765.1 hypothetical protein -
HV012_RS23650 69618..69857 - 240 WP_023356286.1 DNA polymerase III subunit theta -
HV012_RS23655 69860..70060 - 201 WP_032145427.1 hypothetical protein -
HV012_RS23660 70172..70930 - 759 WP_032353390.1 hypothetical protein -
HV012_RS23665 70927..71163 - 237 Protein_71 hypothetical protein -
HV012_RS23670 71170..71867 - 698 Protein_72 IS1 family transposase -
HV012_RS23675 71892..72077 - 186 Protein_73 hypothetical protein -
HV012_RS23680 72084..72377 - 294 WP_000269001.1 hypothetical protein -
HV012_RS23685 72556..72789 - 234 WP_000517421.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..98816 98816
- flank IS/Tn - - 71592..71867 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T164599 WP_001216045.1 NZ_CP056963:c67801-67421 [Escherichia fergusonii]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T164599 NZ_CP072990:c3932570-3932436 [Xanthomonas translucens pv. translucens]
ATGAAGCGTACTTTCGCGTGGATGCTGCTGGCGATGTTCTCGGTGGGCCTGCTGTCGGGCTGCAACACGGTTGCCGGTGC
CGGCAAGGACGTGAAGAGCGCCGGCGAGAAGGTCGAAGACGCCGCCAAGAACTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT164599 WP_001190712.1 NZ_CP056963:c68022-67801 [Escherichia fergusonii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT164599 NZ_CP072990:c3932821-3932669 [Xanthomonas translucens pv. translucens]
ATGAAGCGACTGTTCACCTTGACGATGCTGACCCTGTTTTGTGCAGGCATGCTGACCGGCTGCAACACCATGGCCGGCGC
CGGTAAGGACATGCAGAAGGCCGGCGATAAGGTGGAAACCAAGGCCGACGACTGTAAAGACGGCAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References