Detailed information of TA system TA164598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/FR47-DUF1778
• Location: 41518..42316
• Replicon: plasmid pRHB41-C20_2
• Accession: NZ_CP056962
• Organism: Escherichia fergusonii strain RHB41-C20

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: HV012_RS22925
• Protein ID: WP_077580870.1
• Coordinates: 41795..42316 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: HV012_RS22920
• Protein ID: WP_001711191.1
• Coordinates: 41518..41787 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV012_RS22895	36945..38285	-	1341	WP_087904724.1	AAA family ATPase	-
HV012_RS22900	38346..39071	-	726	WP_097418314.1	hypothetical protein	-
HV012_RS22905	39354..40121	+	768	WP_001711193.1	hypothetical protein	-
HV012_RS22910	40174..40527	-	354	WP_161953766.1	hypothetical protein	-
HV012_RS22915	40533..41198	-	666	WP_023135658.1	AAA family ATPase	-
HV012_RS22920	41518..41787	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
HV012_RS22925	41795..42316	+	522	WP_077580870.1	GNAT family N-acetyltransferase	Toxin
HV012_RS22930	42485..42736	-	252	WP_023135660.1	hypothetical protein	-
HV012_RS22935	42739..43431	-	693	WP_024172707.1	membrane protein	-
HV012_RS22940	43445..43768	-	324	WP_077580871.1	hypothetical protein	-
HV012_RS22945	43902..44483	-	582	WP_077580872.1	tail fiber assembly protein	-
HV012_RS22950	44492..45395	-	904	Protein_46	macro domain-containing protein	-
HV012_RS22955	45392..46348	-	957	Protein_47	tail fiber protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106982	106982

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19428.29 Da
• Isoelectric Point: 8.6369

>T164598 WP_077580870.1 NZ_CP056962:41795-42316 [Escherichia fergusonii]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKRLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T164598 NZ_CP072990:3067153-3067410 [Xanthomonas translucens pv. translucens]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACGACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACGGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACACCGCATCGTCTACAAGGTCGAGGCGGGCGTCCTACTGATCGCGCAGGTG
CGGTATCACTACGCGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT164598 WP_001711191.1 NZ_CP056962:41518-41787 [Escherichia fergusonii]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT164598 NZ_CP072990:3066905-3067156 [Xanthomonas translucens pv. translucens]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGTGTCGTCAACAATCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTGCGCAGCCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTAGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2