Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3405114..3405768 Replicon chromosome
Accession NZ_CP056951
Organism Escherichia fergusonii strain RHB42-C01

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HV015_RS16345 Protein ID WP_000244781.1
Coordinates 3405114..3405521 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HV015_RS16350 Protein ID WP_000354046.1
Coordinates 3405502..3405768 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV015_RS16325 3401071..3402804 - 1734 WP_000813234.1 single-stranded-DNA-specific exonuclease RecJ -
HV015_RS16330 3402810..3403520 - 711 WP_046076902.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HV015_RS16335 3403545..3404441 - 897 WP_046076901.1 site-specific tyrosine recombinase XerD -
HV015_RS16340 3404553..3405074 + 522 WP_001055874.1 flavodoxin FldB -
HV015_RS16345 3405114..3405521 - 408 WP_000244781.1 protein YgfX Toxin
HV015_RS16350 3405502..3405768 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HV015_RS16355 3406020..3407000 + 981 WP_046076900.1 tRNA-modifying protein YgfZ -
HV015_RS16360 3407077..3407736 - 660 WP_000250283.1 hemolysin III family protein -
HV015_RS16365 3407900..3408211 - 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -
HV015_RS16370 3408256..3409689 + 1434 WP_181575963.1 6-phospho-beta-glucosidase BglA -
HV015_RS16375 3409737..3410630 - 894 WP_181576133.1 transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T164557 WP_000244781.1 NZ_CP056951:c3405521-3405114 [Escherichia fergusonii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T164557 NZ_CP072980:c4156679-4156425 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT164557 WP_000354046.1 NZ_CP056951:c3405768-3405502 [Escherichia fergusonii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT164557 NZ_CP072980:c4156927-4156676 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References