Detailed information of TA system TA164556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3129096..3129715
• Replicon: chromosome
• Accession: NZ_CP056951
• Organism: Escherichia fergusonii strain RHB42-C01

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: HV015_RS14990
• Protein ID: WP_001280991.1
• Coordinates: 3129497..3129715 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV015_RS14985
• Protein ID: WP_104917851.1
• Coordinates: 3129096..3129470 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV015_RS14975	3124228..3125421	+	1194	WP_002431407.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HV015_RS14980	3125444..3128593	+	3150	WP_001132493.1	multidrug efflux RND transporter permease subunit	-
HV015_RS14985	3129096..3129470	+	375	WP_104917851.1	Hha toxicity modulator TomB	Antitoxin
HV015_RS14990	3129497..3129715	+	219	WP_001280991.1	hemolysin expression modulator Hha	Toxin
HV015_RS14995	3130212..3130682	+	471	WP_002431406.1	YlaC family protein	-
HV015_RS15000	3130821..3132371	+	1551	WP_001260376.1	EAL domain-containing protein	-
HV015_RS15005	3132413..3132766	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
HV015_RS15015	3133145..3133456	+	312	WP_000409911.1	MGMT family protein	-
HV015_RS15020	3133487..3134059	-	573	WP_000779831.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T164556 WP_001280991.1 NZ_CP056951:3129497-3129715 [Escherichia fergusonii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T164556 NZ_CP072980:4145518-4145892 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14511.31 Da
• Isoelectric Point: 4.8989

>AT164556 WP_104917851.1 NZ_CP056951:3129096-3129470 [Escherichia fergusonii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT164556 NZ_CP072980:4145061-4145429 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• PDB: 4ICG