Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 859833..860416 Replicon chromosome
Accession NZ_CP056916
Organism Escherichia fergusonii strain RHB42-C24

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag HV025_RS04210 Protein ID WP_000254750.1
Coordinates 860081..860416 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HV025_RS04205 Protein ID WP_000581937.1
Coordinates 859833..860081 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV025_RS04195 856172..857470 + 1299 WP_000046872.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
HV025_RS04200 857521..859755 + 2235 WP_046076126.1 GTP diphosphokinase -
HV025_RS04205 859833..860081 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HV025_RS04210 860081..860416 + 336 WP_000254750.1 endoribonuclease MazF Toxin
HV025_RS04215 860487..861278 + 792 WP_001071671.1 nucleoside triphosphate pyrophosphohydrolase -
HV025_RS04220 861506..863143 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HV025_RS04225 863231..864529 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T164501 WP_000254750.1 NZ_CP056916:860081-860416 [Escherichia fergusonii]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T164501 NZ_CP072961:c2841118-2841015 [Enterobacter sp. MGH 14]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT164501 WP_000581937.1 NZ_CP056916:859833-860081 [Escherichia fergusonii]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT164501 NZ_CP072961:2841012-2841211 [Enterobacter sp. MGH 14]
GATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGG
CATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTAAAAGTGGTCT
GAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References