Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 6633..7158 Replicon plasmid pRHBSTW-00006_2
Accession NZ_CP056911
Organism Citrobacter freundii strain RHBSTW-00006

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag HV030_RS25260 Protein ID WP_008322213.1
Coordinates 6853..7158 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag HV030_RS25255 Protein ID WP_032934863.1
Coordinates 6633..6851 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV030_RS25235 1684..2694 - 1011 WP_011191343.1 zinc-binding alcohol dehydrogenase family protein -
HV030_RS25240 3300..3905 + 606 WP_033982388.1 sulfite oxidase-like oxidoreductase -
HV030_RS25245 4103..4800 + 698 Protein_6 IS1 family transposase -
HV030_RS25250 4947..5921 + 975 WP_181219984.1 sensor domain-containing diguanylate cyclase -
HV030_RS25255 6633..6851 + 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HV030_RS25260 6853..7158 + 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
HV030_RS25265 7160..7474 + 315 WP_008322211.1 hypothetical protein -
HV030_RS25270 7464..8255 + 792 WP_008322208.1 site-specific integrase -
HV030_RS25275 8454..9821 - 1368 WP_181638753.1 GntP family transporter -
HV030_RS25280 9907..10683 - 777 WP_008322204.1 HPr family phosphocarrier protein -
HV030_RS25285 10688..11326 - 639 WP_008322203.1 aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..118265 118265
- flank IS/Tn - - 4423..4800 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T164498 WP_008322213.1 NZ_CP056911:6853-7158 [Citrobacter freundii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T164498 NZ_CP072961:c1095548-1095330 [Enterobacter sp. MGH 14]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT164498 WP_032934863.1 NZ_CP056911:6633-6851 [Citrobacter freundii]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT164498 NZ_CP072961:c1095950-1095576 [Enterobacter sp. MGH 14]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References