Detailed information of TA system TA164496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4769353..4769888
• Replicon: chromosome
• Accession: NZ_CP056910
• Organism: Citrobacter freundii strain RHBSTW-00006

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HV030_RS23335
• Protein ID: WP_117343582.1
• Coordinates: 4769601..4769888 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D4BKM9
• Locus tag: HV030_RS23330
• Protein ID: WP_006688247.1
• Coordinates: 4769353..4769604 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV030_RS23300	4764366..4765214	+	849	WP_135912711.1	alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase PhnJ	-
HV030_RS23305	4765211..4765969	+	759	WP_181638374.1	phosphonate C-P lyase system protein PhnK	-
HV030_RS23310	4766077..4766757	+	681	WP_117343579.1	phosphonate C-P lyase system protein PhnL	-
HV030_RS23315	4766754..4767890	+	1137	WP_096758663.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
HV030_RS23320	4767893..4768438	+	546	WP_096758664.1	ribose 1,5-bisphosphokinase	-
HV030_RS23325	4768511..4769269	+	759	WP_181540129.1	phosphonate metabolism protein PhnP	-
HV030_RS23330	4769353..4769604	+	252	WP_006688247.1	plasmid stabilization protein	Antitoxin
HV030_RS23335	4769601..4769888	+	288	WP_117343582.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV030_RS23340	4769885..4770214	-	330	WP_096758667.1	hypothetical protein	-
HV030_RS23345	4770283..4772547	-	2265	WP_117343583.1	hybrid sensor histidine kinase/response regulator	-
HV030_RS23350	4772662..4774185	+	1524	WP_181638375.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11139.00 Da
• Isoelectric Point: 10.2830

>T164496 WP_117343582.1 NZ_CP056910:4769601-4769888 [Citrobacter freundii]
MSYTVKFREDALKEWQKLDKTIQQQLAKELKKCGENPHVPSAKLRGIKDCYKIQLRTSRFRLVYQVIDDTLVIAVVAVGK
RERSEVYNLASERLR

Nucleotide

• Length: 288 bp

>T164496 NZ_CP072961:c298678-298376 [Enterobacter sp. MGH 14]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTGGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9215.53 Da
• Isoelectric Point: 4.2965

>AT164496 WP_006688247.1 NZ_CP056910:4769353-4769604 [Citrobacter freundii]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQQLVKLVHERSQQPLVDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT164496 NZ_CP072961:c298369-298082 [Enterobacter sp. MGH 14]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7X1BT32