Detailed information of TA system TA164494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4511331..4511847
• Replicon: chromosome
• Accession: NZ_CP056910
• Organism: Citrobacter freundii strain RHBSTW-00006

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5I6CHB9
• Locus tag: HV030_RS22040
• Protein ID: WP_000220581.1
• Coordinates: 4511331..4511615 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HV030_RS22045
• Protein ID: WP_115259576.1
• Coordinates: 4511605..4511847 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV030_RS22025	4506532..4508184	+	1653	WP_181540093.1	alpha,alpha-phosphotrehalase	-
HV030_RS22030	4508603..4510741	+	2139	WP_115259574.1	anaerobic ribonucleoside-triphosphate reductase	-
HV030_RS22035	4510863..4511327	+	465	WP_096759476.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
HV030_RS22040	4511331..4511615	-	285	WP_000220581.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV030_RS22045	4511605..4511847	-	243	WP_115259576.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HV030_RS22050	4511925..4513838	-	1914	WP_117342405.1	BglG family transcription antiterminator	-
HV030_RS22055	4513860..4514600	-	741	WP_115259578.1	KDGP aldolase family protein	-
HV030_RS22060	4514597..4515715	-	1119	WP_096758517.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
HV030_RS22065	4515699..4516832	-	1134	WP_146717608.1	amidohydrolase/deacetylase family metallohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10868.70 Da
• Isoelectric Point: 10.0482

>T164494 WP_000220581.1 NZ_CP056910:c4511615-4511331 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQLKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVIVFVVAVGK
REHSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T164494 NZ_CP072954:c240123-239593 [Enterobacter sp. MGH 3]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8947.32 Da
• Isoelectric Point: 5.0490

>AT164494 WP_115259576.1 NZ_CP056910:c4511847-4511605 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLDKLNITPTEAVRLLFQYVAETGRMPVKTVTLSDSEDALIQTVRERLSSPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT164494 NZ_CP072954:c240396-240127 [Enterobacter sp. MGH 3]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A5I6CHB9