Detailed information of TA system TA164493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4435047..4435623
• Replicon: chromosome
• Accession: NZ_CP056910
• Organism: Citrobacter freundii strain RHBSTW-00006

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HV030_RS21695
• Protein ID: WP_117342369.1
• Coordinates: 4435336..4435623 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HV030_RS21690
• Protein ID: WP_117342368.1
• Coordinates: 4435047..4435349 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV030_RS21675	4431515..4433665	+	2151	WP_135912629.1	pyruvate/proton symporter BtsT	-
HV030_RS21680	4433773..4433967	+	195	WP_003830038.1	YbdD/YjiX family protein	-
HV030_RS21685	4433980..4434936	+	957	WP_146717580.1	GTPase	-
HV030_RS21690	4435047..4435349	-	303	WP_117342368.1	BrnA antitoxin family protein	Antitoxin
HV030_RS21695	4435336..4435623	-	288	WP_117342369.1	BrnT family toxin	Toxin
HV030_RS21700	4435857..4436507	+	651	Protein_4256	restriction endonuclease	-
HV030_RS21705	4436530..4437186	+	657	WP_181625597.1	restriction endonuclease subunit S	-
HV030_RS21710	4437402..4437746	+	345	WP_181540063.1	endoribonuclease SymE	-
HV030_RS21715	4437864..4438028	-	165	WP_117342370.1	DUF1127 domain-containing protein	-
HV030_RS21720	4438205..4439617	+	1413	WP_181625598.1	PLP-dependent aminotransferase family protein	-
HV030_RS21725	4439644..4440594	-	951	WP_181638343.1	Rpn family recombination-promoting nuclease/putative transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (42-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11362.75 Da
• Isoelectric Point: 7.5239

>T164493 WP_117342369.1 NZ_CP056910:c4435623-4435336 [Citrobacter freundii]
MPMEFEWDANKAQSNHRKHGVRFEDAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHD

Nucleotide

• Length: 288 bp

>T164493 NZ_CP072954:c124422-124006 [Enterobacter sp. MGH 3]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCTGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCAGTGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGATGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11444.95 Da
• Isoelectric Point: 8.5366

>AT164493 WP_117342368.1 NZ_CP056910:c4435349-4435047 [Citrobacter freundii]
MSMIKHKRGSASALSAQHEAELKALANKPDDDIDYSDIPVTEDEQWSEANRGTFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT164493 NZ_CP072954:c124649-124419 [Enterobacter sp. MGH 3]
ATGCGCACAGTTTCTATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCATTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA