Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3656395..3656921 Replicon chromosome
Accession NZ_CP056910
Organism Citrobacter freundii strain RHBSTW-00006

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HV030_RS17990 Protein ID WP_000323025.1
Coordinates 3656634..3656921 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HV030_RS17985 Protein ID WP_000534858.1
Coordinates 3656395..3656634 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV030_RS17970 3652770..3653930 - 1161 WP_060598734.1 IS30-like element ISCfr4 family transposase -
HV030_RS17975 3654437..3655009 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
HV030_RS17980 3655209..3656132 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
HV030_RS17985 3656395..3656634 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HV030_RS17990 3656634..3656921 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HV030_RS17995 3657118..3658083 + 966 WP_049269752.1 lipoyl synthase -
HV030_RS18000 3658181..3658384 - 204 WP_045441030.1 twin-arginine translocase subunit TatE -
HV030_RS18005 3658513..3659301 - 789 WP_117342217.1 deaminated glutathione amidase -
HV030_RS18010 3659392..3659775 + 384 WP_096757905.1 fluoride efflux transporter CrcB -
HV030_RS18015 3659827..3660036 - 210 WP_000034826.1 transcription antiterminator/RNA stability regulator CspE -
HV030_RS18020 3660225..3660788 - 564 WP_117342216.1 lipid IV(A) palmitoyltransferase PagP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T164490 WP_000323025.1 NZ_CP056910:3656634-3656921 [Citrobacter freundii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T164490 NZ_CP072953:c2841119-2841016 [Enterobacter sp. MGH 3]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT164490 WP_000534858.1 NZ_CP056910:3656395-3656634 [Citrobacter freundii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT164490 NZ_CP072953:2841013-2841212 [Enterobacter sp. MGH 3]
GATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGG
CATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTAAAAGTGGTCT
GAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References