Detailed information of TA system TA164468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2028..2563
• Replicon: plasmid pRHBSTW-00011_3
• Accession: NZ_CP056909
• Organism: Citrobacter freundii strain RHBSTW-00011

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T1PX77
• Locus tag: HV032_RS26110
• Protein ID: WP_001523468.1
• Coordinates: 2276..2563 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1PXV2
• Locus tag: HV032_RS26105
• Protein ID: WP_001139206.1
• Coordinates: 2028..2279 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV032_RS26100	46..1077	+	1032	WP_080182593.1	replication initiation protein	-
HV032_RS26105	2028..2279	+	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HV032_RS26110	2276..2563	+	288	WP_001523468.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV032_RS26115	2573..3118	+	546	WP_001523466.1	hypothetical protein	-
HV032_RS26120	3604..4191	+	588	WP_024229909.1	serine acetyltransferase	-
HV032_RS26125	4470..5063	-	594	WP_001523457.1	hypothetical protein	-
HV032_RS26130	6066..6830	+	765	WP_021013229.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..95042	95042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11142.18 Da
• Isoelectric Point: 10.6741

>T164468 WP_001523468.1 NZ_CP056909:2276-2563 [Citrobacter freundii]
MTYTVKFRDDALREWLRLDKAIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Nucleotide

• Length: 288 bp

>T164468 NZ_CP072946:c336294-335836 [Enterobacter sp. MGH 7]
GTGATCCTGTATCGGATGACCAAGACCCGCTATATTACGACTGCATGGTCGGGATTAGGTGCGAAAGAAGCGGGAGGCCG
CTGGAACAGTGTCGGCACAGCACTTGTCTATCTGTCCGAGACGGCCTCTCTGACCATGCTGGAGACGCTGGTGCATATCA
ATGCCCCGCAGCTTCTGGACGCTTACACGCTGCTGAGCATTGATGTCCCGGATGACCAGATCCAGGCCTTTGACTTGAAC
CTGCTGCCGCCGGGCTGGGCAAGTGAAGATGCGCCCGCAGAACTTGCCCTGTATGGCGATAACTGGGCTGAAAGTGGCTC
TTCTGTTGCCCTGCGTATTCCCAGTGCATTATCTCCGGTGGAGTTTAACTACCTTTTAAATCCGGCTCATCCGGCGATTT
TTGATCTGATGAAAACCGTCCAGAAAATTCCTTACCAGTACGACACACGGCTGAAATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9171.39 Da
• Isoelectric Point: 4.0927

>AT164468 WP_001139206.1 NZ_CP056909:2028-2279 [Citrobacter freundii]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT164468 NZ_CP072946:c336737-336291 [Enterobacter sp. MGH 7]
ATGACTATGAAAGTTTTTTCTCCAGATTTATCCAAAATGAACCCGCACGGCTTATGGCAGGTGGTGGGTCTGGATAATGA
CGGCATTGCGCTGATGGACAAAATTAGCCACGGACTGGAAGGTAAAGTCGCTCATTCCATTTCTGAATGGGCGAACATCA
CGCCGTCGGAACTGCGCAAAATGTCCGGCATTCCGAACACCACCTTCAATCGCAGCGTAAAAGCCCGTTTCACTGCGGAT
CAGAGCGAACGTCTGGTGCGTATTATTCGCGTCATTGAACGGGCTGTAGAGCTGTTCGAAGGCGACAAAGAGGCAGCACA
GAAATGGATGAACGAGCCAAACCGTGGCTTGAGCTGGAAGACTCCGGCAGATGTTGTCTCTTCGGAAACCGGCGCACTCG
AAGTGATGCGCCTGATCACCCGTATTGAACACGGAGTCTATTCGTGA

Structures

Toxin

• AlphaFold DB: T1PX77

Antitoxin

• AlphaFold DB: T1PXV2