Detailed information of TA system TA164467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5202629..5203245
• Replicon: chromosome
• Accession: NZ_CP056907
• Organism: Citrobacter freundii strain RHBSTW-00011

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV032_RS24700
• Protein ID: WP_172862540.1
• Coordinates: 5202629..5203003 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6B5NTK4
• Locus tag: HV032_RS24705
• Protein ID: WP_043018956.1
• Coordinates: 5203003..5203245 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV032_RS24685	5200132..5201034	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
HV032_RS24690	5201031..5201666	+	636	WP_043018888.1	formate dehydrogenase cytochrome b556 subunit	-
HV032_RS24695	5201663..5202592	+	930	WP_043018887.1	formate dehydrogenase accessory protein FdhE	-
HV032_RS24700	5202629..5203003	-	375	WP_172862540.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV032_RS24705	5203003..5203245	-	243	WP_043018956.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV032_RS24710	5203451..5204359	+	909	WP_181634181.1	alpha/beta hydrolase	-
HV032_RS24715	5204424..5204666	+	243	WP_003825282.1	type II toxin-antitoxin system ParD family antitoxin	-
HV032_RS24720	5204659..5204946	+	288	WP_003825284.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HV032_RS24725	5204952..5205893	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HV032_RS24730	5205938..5206375	-	438	WP_003028671.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV032_RS24735	5206372..5207244	-	873	WP_043018884.1	virulence factor BrkB family protein	-
HV032_RS24740	5207238..5207837	-	600	WP_043018883.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13589.83 Da
• Isoelectric Point: 8.5950

>T164467 WP_172862540.1 NZ_CP056907:c5203003-5202629 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLALETWPPQNAISLITWMEVMVGAKKYHQEALTRVALGAFNVIGISQDIAERSVSI
RQEHGMKLPDAIILATAQIHRLTLVTRNTKDFAGISGVVTPYAL

Nucleotide

• Length: 375 bp

>T164467 NZ_CP072946:c279930-279643 [Enterobacter sp. MGH 7]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9451.75 Da
• Isoelectric Point: 4.3437

>AT164467 WP_043018956.1 NZ_CP056907:c5203245-5203003 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT164467 NZ_CP072946:c280169-279930 [Enterobacter sp. MGH 7]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6B5NTK4