Detailed information of TA system TA164464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4613392..4613968
• Replicon: chromosome
• Accession: NZ_CP056907
• Organism: Citrobacter freundii strain RHBSTW-00011

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HV032_RS22040
• Protein ID: WP_075145248.1
• Coordinates: 4613681..4613968 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HV032_RS22035
• Protein ID: WP_043018311.1
• Coordinates: 4613392..4613694 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV032_RS22020	4609852..4612002	+	2151	WP_075145247.1	pyruvate/proton symporter BtsT	-
HV032_RS22025	4612117..4612311	+	195	WP_003830038.1	YbdD/YjiX family protein	-
HV032_RS22030	4612324..4613280	+	957	WP_043018312.1	GTPase	-
HV032_RS22035	4613392..4613694	-	303	WP_043018311.1	BrnA antitoxin family protein	Antitoxin
HV032_RS22040	4613681..4613968	-	288	WP_075145248.1	BrnT family toxin	Toxin
HV032_RS22045	4614122..4615186	-	1065	WP_043018309.1	DUF2955 domain-containing protein	-
HV032_RS22050	4615176..4616243	-	1068	WP_181634100.1	HlyD family secretion protein	-
HV032_RS22055	4616250..4616714	-	465	WP_043018307.1	MarR family transcriptional regulator	-
HV032_RS22060	4616860..4617024	-	165	WP_080625765.1	DUF1127 domain-containing protein	-
HV032_RS22065	4617202..4618614	+	1413	WP_080625766.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11219.68 Da
• Isoelectric Point: 8.5380

>T164464 WP_075145248.1 NZ_CP056907:c4613968-4613681 [Citrobacter freundii]
MPMEFVWDANKAQSNHRKHGVRFEDAVLVFDDPRHLSRQDRYENGEYRWQTIGMMYGVVVILVAHSVRFESGVEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T164464 NZ_CP072945:c2875634-2875531 [Enterobacter sp. MGH 7]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11485.10 Da
• Isoelectric Point: 10.1293

>AT164464 WP_043018311.1 NZ_CP056907:c4613694-4613392 [Citrobacter freundii]
MSMVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEANRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT164464 NZ_CP072945:2875528-2875727 [Enterobacter sp. MGH 7]
GATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGG
CATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTAAAAGTGGTCT
GAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC