Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4009275..4009895 Replicon chromosome
Accession NZ_CP056907
Organism Citrobacter freundii strain RHBSTW-00011

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV032_RS19280 Protein ID WP_002892050.1
Coordinates 4009677..4009895 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV032_RS19275 Protein ID WP_003021733.1
Coordinates 4009275..4009649 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV032_RS19265 4004421..4005614 + 1194 WP_043015418.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV032_RS19270 4005637..4008786 + 3150 WP_043015417.1 multidrug efflux RND transporter permease subunit -
HV032_RS19275 4009275..4009649 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV032_RS19280 4009677..4009895 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV032_RS19285 4010076..4010627 + 552 WP_043015416.1 maltose O-acetyltransferase -
HV032_RS19290 4010744..4011214 + 471 WP_043015415.1 YlaC family protein -
HV032_RS19295 4011294..4011434 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV032_RS19300 4011436..4011696 - 261 WP_043015414.1 type B 50S ribosomal protein L31 -
HV032_RS19305 4011885..4013438 + 1554 WP_172862784.1 EAL domain-containing protein -
HV032_RS19310 4013490..4013843 - 354 WP_003831033.1 DUF1428 family protein -
HV032_RS19315 4013908..4014537 - 630 WP_043015412.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T164463 WP_002892050.1 NZ_CP056907:4009677-4009895 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T164463 NZ_CP072945:c2440924-2440439 [Enterobacter sp. MGH 7]
GTGGGACGTGTAACAGCGCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCATTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCACTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATACTTGCCCGTCTGGCTGTTGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCCTCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT164463 WP_003021733.1 NZ_CP056907:4009275-4009649 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT164463 NZ_CP072945:c2441178-2440912 [Enterobacter sp. MGH 7]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References