Detailed information of TA system TA164462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3226005..3226595
• Replicon: chromosome
• Accession: NZ_CP056907
• Organism: Citrobacter freundii strain RHBSTW-00011

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HV032_RS15705
• Protein ID: WP_172862932.1
• Coordinates: 3226005..3226337 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: HV032_RS15710
• Protein ID: WP_172862931.1
• Coordinates: 3226338..3226595 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV032_RS15680	3222056..3222775	+	720	WP_043015969.1	HAD family phosphatase	-
HV032_RS15685	3222793..3223941	+	1149	WP_075146310.1	MFS transporter	-
HV032_RS15695	3224119..3225762	+	1644	WP_141110908.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HV032_RS15705	3226005..3226337	-	333	WP_172862932.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HV032_RS15710	3226338..3226595	-	258	WP_172862931.1	antitoxin	Antitoxin
HV032_RS15715	3226943..3227149	-	207	WP_172862930.1	helix-turn-helix domain-containing protein	-
HV032_RS15720	3227146..3227607	-	462	WP_181633890.1	hypothetical protein	-
HV032_RS15725	3227495..3227824	-	330	WP_181634731.1	ash family protein	-
HV032_RS15730	3228147..3230567	+	2421	WP_181633891.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3218092..3251879	33787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11690.60 Da
• Isoelectric Point: 10.7301

>T164462 WP_172862932.1 NZ_CP056907:c3226337-3226005 [Citrobacter freundii]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKAAFNKLTRLPVVVPVTSGGNFARAAGFAVSLEGAGTKTTGVIRCDQPRTI
DMSARNGIRLERIPAAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T164462 NZ_CP072945:c1264918-1264700 [Enterobacter sp. MGH 7]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9456.94 Da
• Isoelectric Point: 4.3690

>AT164462 WP_172862931.1 NZ_CP056907:c3226595-3226338 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIENGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKELL

Nucleotide

• Length: 258 bp

>AT164462 NZ_CP072945:c1265320-1264946 [Enterobacter sp. MGH 7]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA