Detailed information of TA system TA164451

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 910213..910867
• Replicon: chromosome
• Accession: NZ_CP056907
• Organism: Citrobacter freundii strain RHBSTW-00011

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: HV032_RS04345
• Protein ID: WP_043017506.1
• Coordinates: 910460..910867 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: HV032_RS04340
• Protein ID: WP_016154349.1
• Coordinates: 910213..910479 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV032_RS04315	905416..906849	-	1434	WP_043017510.1	6-phospho-beta-glucosidase BglA	-
HV032_RS04320	906969..907697	-	729	WP_008785744.1	MurR/RpiR family transcriptional regulator	-
HV032_RS04325	907750..908061	+	312	WP_043017509.1	N(4)-acetylcytidine aminohydrolase	-
HV032_RS04330	908225..908884	+	660	WP_043017508.1	hemolysin III family protein	-
HV032_RS04335	908976..909956	-	981	WP_047408911.1	tRNA-modifying protein YgfZ	-
HV032_RS04340	910213..910479	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
HV032_RS04345	910460..910867	+	408	WP_043017506.1	protein YgfX	Toxin
HV032_RS04350	910967..911488	-	522	WP_181634333.1	flavodoxin FldB	-
HV032_RS04355	911602..912498	+	897	WP_003825519.1	site-specific tyrosine recombinase XerD	-
HV032_RS04360	912522..913235	+	714	WP_100194819.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV032_RS04365	913241..914974	+	1734	WP_172862381.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15891.76 Da
• Isoelectric Point: 11.5692

>T164451 WP_043017506.1 NZ_CP056907:910460-910867 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMNGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDVGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T164451 NZ_CP072934:c331270-330977 [Corynebacterium pseudogenitalium]
GTGAAGGAAGTAACATTTTCAGCGTTCGATGCAAGCAAATATCTCGATAGCCAAGAAGCAGTGAACGATTACCTGGCTAT
CGCACTCGAAGACGGCGATACGAAAACCGTACAGGTGGTTTTGCGTGACATTGCAAGGGCACACGGCATGAGCCAGCTCG
CTAAAGAGACTCAACTCAACCGTGAATCCCTCTACAAGTCACTATCAAAAGACGGTAACCCGTCCTTTGCGGCTATCACG
AAAATCATGAAAGCCCTGGGACTCAAAATAACTCTCGCTACACAAAATGCCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT164451 WP_016154349.1 NZ_CP056907:910213-910479 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT164451 NZ_CP072934:c331575-331267 [Corynebacterium pseudogenitalium]
GTGGAGATCATTTCCAGCAGCTTGTTTGACGCCTGGTTAGGTGAGCTAAAAGACAAACATGCTCAGCGACGCATTTTGTA
TGCAATAGCCCGATGCGAAACACACGGAAGAATGATTGGAGACATCAAACCCGTTGGCGAGAAGGTTAGCGAGATGAGAT
TTCATTTCGGGCCCGGCTATCGGGTGTACTACACCCGACTAGGCGCAATAACGGTTTTCCTGCTGGCCGGAGGAGATAAA
TCCAGCCAGGCTAAAGATATTCGAACCGCTCAAAAGCTCGCACAACAGGTAAGGGAGGAACAGTCGTGA

Structures

Antitoxin

• AlphaFold DB: R8WMJ6