Detailed information of TA system TA164446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3712156..3712776
• Replicon: chromosome
• Accession: NZ_CP056899
• Organism: Citrobacter sp. RHBSTW-00017

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: HV037_RS18050
• Protein ID: WP_002892050.1
• Coordinates: 3712558..3712776 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV037_RS18045
• Protein ID: WP_181500127.1
• Coordinates: 3712156..3712530 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV037_RS18035	3707303..3708496	+	1194	WP_016152074.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HV037_RS18040	3708519..3711668	+	3150	WP_016152073.1	multidrug efflux RND transporter permease subunit	-
HV037_RS18045	3712156..3712530	+	375	WP_181500127.1	Hha toxicity modulator TomB	Antitoxin
HV037_RS18050	3712558..3712776	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
HV037_RS18055	3712957..3713508	+	552	WP_103283555.1	maltose O-acetyltransferase	-
HV037_RS18060	3713625..3714095	+	471	WP_016152071.1	YlaC family protein	-
HV037_RS18065	3714173..3714313	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
HV037_RS18070	3714315..3714575	-	261	WP_016152070.1	type B 50S ribosomal protein L31	-
HV037_RS18075	3714764..3716317	+	1554	WP_047416867.1	EAL domain-containing protein	-
HV037_RS18080	3716369..3716722	-	354	WP_003831033.1	DUF1428 family protein	-
HV037_RS18085	3716787..3717416	-	630	WP_103283554.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T164446 WP_002892050.1 NZ_CP056899:3712558-3712776 [Citrobacter sp. RHBSTW-00017]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T164446 NZ_CP072933:1346850-1347146 [Xylella fastidiosa subsp. multiplex]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATATCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14445.26 Da
• Isoelectric Point: 5.5653

>AT164446 WP_181500127.1 NZ_CP056899:3712156-3712530 [Citrobacter sp. RHBSTW-00017]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKMIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT164446 NZ_CP072933:1347150-1347455 [Xylella fastidiosa subsp. multiplex]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAACTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTATCGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2