Detailed information of TA system TA164436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2508925..2509561
• Replicon: chromosome
• Accession: NZ_CP056899
• Organism: Citrobacter sp. RHBSTW-00017

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: HV037_RS12135
• Protein ID: WP_049259794.1
• Coordinates: 2508925..2509113 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV037_RS12140
• Protein ID: WP_146053235.1
• Coordinates: 2509145..2509561 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV037_RS12120	2505785..2506345	-	561	WP_001161490.1	recombinase family protein	-
HV037_RS12125	2506736..2507269	-	534	Protein_2375	cytochrome d ubiquinol oxidase subunit II	-
HV037_RS12130	2507269..2508661	-	1393	Protein_2376	cytochrome ubiquinol oxidase subunit I	-
HV037_RS12135	2508925..2509113	+	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV037_RS12140	2509145..2509561	+	417	WP_146053235.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV037_RS12145	2509654..2511063	+	1410	WP_103283798.1	PLP-dependent aminotransferase family protein	-
HV037_RS12150	2511393..2512538	+	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
HV037_RS12155	2512555..2513571	+	1017	WP_016153153.1	ABC transporter ATP-binding protein	-
HV037_RS12160	2513572..2514516	+	945	WP_016153152.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T164436 WP_049259794.1 NZ_CP056899:2508925-2509113 [Citrobacter sp. RHBSTW-00017]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T164436 NZ_CP072932:c732379-732092 [Xylella fastidiosa subsp. multiplex]
ATGGCTAAGTCCTATCGACTGACACCGCTGGCCGAGGCCGATCTTGAAGAAATCTGGTTCTACACATTCAGGCACTGGTC
GATAGAGCAGGCCGACAGCTACCACCGTAGCCTAGTTGCAGTTTTCGAGGGGCTGGCAGCAGGTACAAAGCAAGGCTGCC
CTTCTGTTTTGACGGACTTCAATAAATACCTATGTGGTTCGCATGTAGTGTATTTCATGGATTACGCCGATCACTTGGAT
ATTATCCGCATCCTGCATCAGCGGCAGGATACCGAGCGGTACCTATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15080.33 Da
• Isoelectric Point: 4.7119

>AT164436 WP_146053235.1 NZ_CP056899:2509145-2509561 [Citrobacter sp. RHBSTW-00017]
MRYPVNLIPSVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGTEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT164436 NZ_CP072932:c732614-732372 [Xylella fastidiosa subsp. multiplex]
ATGAGTCGAAACACATCCGTTGCCCTCGGGCCGCACTTCACCAGCTTTATTGATGCCCAAGTCCAGGGCGGTCGCTACGG
TACCGCCAGCGATGTTGTCAGGGCCGGTCTGCGGCTGCTTGAGGAACACGAAACAAAGGTGAAGGCACTGCAAGATGCGC
TGAATGTTGGCCACCAATCCGGCGAGCCGCGCCCGTTCGACAGCGAGGTGTTTTTGAGCCGGATGCACACGCAGCATGGC
TAA

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9