Detailed information of TA system TA164434

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 986829..987483
• Replicon: chromosome
• Accession: NZ_CP056899
• Organism: Citrobacter sp. RHBSTW-00017

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: HV037_RS04820
• Protein ID: WP_016154348.1
• Coordinates: 987076..987483 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: HV037_RS04815
• Protein ID: WP_016154349.1
• Coordinates: 986829..987095 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV037_RS04790	982042..983475	-	1434	WP_016154353.1	6-phospho-beta-glucosidase BglA	-
HV037_RS04795	983596..984324	-	729	WP_103284472.1	MurR/RpiR family transcriptional regulator	-
HV037_RS04800	984377..984688	+	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
HV037_RS04805	984852..985511	+	660	WP_016154351.1	hemolysin III family protein	-
HV037_RS04810	985591..986571	-	981	WP_016154350.1	tRNA-modifying protein YgfZ	-
HV037_RS04815	986829..987095	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
HV037_RS04820	987076..987483	+	408	WP_016154348.1	protein YgfX	Toxin
HV037_RS04825	987584..988105	-	522	WP_016154347.1	flavodoxin FldB	-
HV037_RS04830	988219..989115	+	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
HV037_RS04835	989139..989852	+	714	WP_075847456.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV037_RS04840	989858..991591	+	1734	WP_103284192.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T164434 WP_016154348.1 NZ_CP056899:987076-987483 [Citrobacter sp. RHBSTW-00017]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T164434 NZ_CP072931:c2600151-2599825 [Streptomyces auratus AGR0001]
ATGATCGATCTGGAGCCGGCGCGCGGCAGTGAGGCCAACAAGACCCGCCCGGCCGTGATCGTGTCCAACAACGGCGCCAA
TCAGTCGGTCGAGAGCAACCGTCGGGGGGTGGTCACGGTCGTTCCCCTGACGTCGAGCACCTCGCGTGTCCTGACGTTCC
AGGTGTTCCTCGGCGCCGAGGAGTGCCGTCTGCCCAAGGACTGCAAGGCCCAGTGCGAACAGGTCCGCGCCGTCACTGTG
GAGCGCGTGCGCCAACGGGTCGGCTCGGTGCCCAGGCAGCGCATGGTGGAGATCGACGTCGCGCTCCGGCGGCATCTGGC
GCTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT164434 WP_016154349.1 NZ_CP056899:986829-987095 [Citrobacter sp. RHBSTW-00017]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT164434 NZ_CP072931:c2600389-2600159 [Streptomyces auratus AGR0001]
ATGAAGATTAGTGTGAGTCTGCCTCAGGAGGACCTCGCCTTCGTCGACGAGTACGCCACGAGAACGGAGGCGGAGTCCCG
GTCCGCCGTGATACACGCCGCCATCGAACTGCTGCGTGCCGCGGAACTCGAATCGGAGTACACCGAGGCGTTCGAGGAGT
GGGACGCGAGCGAGGATGCGGCGCTCTGGGACCGGACGAGCGGGGATGGGCTTGCCGATGAGGCGCGGTGA

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6