Detailed information of TA system TA164433

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 754409..755088
• Replicon: chromosome
• Accession: NZ_CP056899
• Organism: Citrobacter sp. RHBSTW-00017

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A7D6Z078
• Locus tag: HV037_RS03630
• Protein ID: WP_048215514.1
• Coordinates: 754747..755088 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV037_RS03625
• Protein ID: WP_181545588.1
• Coordinates: 754409..754726 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV037_RS03585	749968..750801	+	834	WP_172748778.1	DUF945 domain-containing protein	-
HV037_RS03590	751003..751707	+	705	WP_046495813.1	WYL domain-containing protein	-
HV037_RS03595	751704..752318	+	615	WP_046495819.1	hypothetical protein	-
HV037_RS03600	752407..752817	+	411	WP_046495825.1	hypothetical protein	-
HV037_RS03605	752895..753131	+	237	WP_046495830.1	DUF905 domain-containing protein	-
HV037_RS03610	753210..753668	+	459	WP_046495834.1	antirestriction protein	-
HV037_RS03615	753684..754160	+	477	WP_046495838.1	RadC family protein	-
HV037_RS03620	754169..754390	+	222	WP_000691995.1	DUF987 domain-containing protein	-
HV037_RS03625	754409..754726	+	318	WP_181545588.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV037_RS03630	754747..755088	+	342	WP_048215514.1	TA system toxin CbtA family protein	Toxin
HV037_RS03635	755204..756037	+	834	WP_046495848.1	DUF4942 domain-containing protein	-
HV037_RS03645	756341..756847	+	507	WP_103284247.1	G/U mismatch-specific DNA glycosylase	-
HV037_RS03650	756893..758737	-	1845	WP_016154549.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	733450..755088	21638

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13014.97 Da
• Isoelectric Point: 10.4182

>T164433 WP_048215514.1 NZ_CP056899:754747-755088 [Citrobacter sp. RHBSTW-00017]
MKPQSAATSRAVKPCLSPVAIWQILLTRLLEQHYGLTLNDTPFRDESVIQEHIDAGITLANAVNFLVEKYELVRIDRRRF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T164433 NZ_CP072916:1737069-1737470 [Comamonas aquatica]
ATGATCCTGCTCGATACCAACATCTGCATCTACATCATCAACGCCAAGCCCCCCACCGTGCTGGCGCGCTTTCAGCAATA
CCGCATGGGCGATGTGGGGCTGTGCAGCGTGGTGGCTGCAGAGCTGGCATTTGGCGTGGCCAAAAGTGGCTCTGCCCGCA
ACCGCCAGGCGCTGGAGATGTTTCTGGCCCCGCTCACCATCTTGCCGTTTGACGATGCTGCCGTGTGGGCCTATGGCGAC
CTGCGCGCCGACCTGGAGCGCCGTGGCACTCCGATTGGCGCACTCGACACCATGATTGCCGCGCACGCCCTGAGCCAACA
GGCCCTGCTGGTCACCAACAACACCCGCGAATTTGCCAAAGTGCCCGGCCTGCAGCTAGACAACTGGGCCGCCGCCACCT
GA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11826.46 Da
• Isoelectric Point: 6.3129

>AT164433 WP_181545588.1 NZ_CP056899:754409-754726 [Citrobacter sp. RHBSTW-00017]
MSNITWGLQRDITPLLGTRLVQEGNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTSGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Nucleotide

• Length: 318 bp

>AT164433 NZ_CP072916:1736839-1737072 [Comamonas aquatica]
ATGGACACCGCACGCCTTTTTCAATCTGGCCGCAGCCAAGCCGTGCGGCTGCCCAAGGAATACCGCTTCACCGGCACGGA
AGTCGTGGTGAAGCACTTTGGCAACGGCGTGCTGCTGCTGCCCGCAGACAACCCCTGGCAAACGCTGCAAGCCGGGCTAG
CCGCTTTTGAGCCCGGCTTTGTGCTCACCCGCGAGCAGCCTGACACCCAGCAGCGGGACGAGATCAACCCATGA

Structures

Toxin

• AlphaFold DB: A0A7D6Z078